安徽医科大学学报2024，Vol.59Issue(2)：310-315,6.DOI:10.19405/j.cnki.issn1000-1492.2024.02.021
蟾毒灵抑制M2型巨噬细胞介导的结直肠癌迁移和上皮间质转化
Inhibition of M2-type macrophage-mediated migration and epithelial mesenchymal transition in colorectal cancer by bufalin
摘要
Abstract
Objective To investigate the role of bufalin(BU)in inhibiting M2-type macrophage-mediated colorec-tal cancer metastasis.Methods Human acute leukemia mononuclear cells(THP-1)were differentiated into M0 macrophages using phorbol ester induction(PMA)for 48 hours.The M0 macrophages were then treated with IL-4 and IL-13 medium.Surface markers and morphological changes were observed through ELISA,morphology,and RT-qPCR experiments.RT-PCR and ELISA experiments were conducted to detect the surface markers TGF-β and IL-10 of M2 macrophages.The secretion level of IL-6 in the supernatant of M2 macrophages and colorectal cancer cells HCT116 was compared using ELISA.Additionally,the effect of conditioned medium on colorectal cancer cell HCT116 was assessed through Transwell,Wound healing,RT-qPCR,and Western blot experiments.Subsequent-ly,bufalin was added to the conditioned medium and the changes in AKT/PI3K protein,migration,and epithelial-mesenchymal transition ability in HCT116 were observed using Western blot,Transwell,Wound healing and RT-qPCR experiments.Results THP-1 were successfully differentiated into M2 macrophages.The activation of AKT/PI3K protein in HCT116 cells was induced by the secretion of IL-6 from M2 macrophages,which in turn promoted the migration and epithelial-mesenchymal transition ability of the HCT116 cells.The migration and epithelial-mes-enchymal transition mediated by M2 macrophages in HCT116 cells were effectively inhibited by Bufalin.Conclu-sion The release of IL-6 from M2 macrophages activates the AKT/PI3K signaling pathway in colorectal cancer cells,thereby promoting their migration and epithelial-mesenchymal transition capacity.Moreover,bufalin exhibits inhibitory effects on this effect.关键词
蟾毒灵/M2型巨噬细胞/结直肠癌Key words
bufalin/M2 macrophage/colorectal cancer分类
医药卫生引用本文复制引用
唐东豪,王杰,陈进宝,贾琳琳,沈东晓,尚靖,冯月娇,卢佳豪,肖增友,何钰洁..蟾毒灵抑制M2型巨噬细胞介导的结直肠癌迁移和上皮间质转化[J].安徽医科大学学报,2024,59(2):310-315,6.基金项目
国家自然科学基金面上项目(编号:81973625) （编号:81973625）
上海市启明星项目(编号:22YF1441400) （编号:22YF1441400）
安徽医科大学研究生科研与实践创新项目资助(编号:YJS20230098) （编号:YJS20230098）
上海市中医医院第三届中医传承人才项目(编号:2023zycc02) （编号:2023zycc02）
