安徽医科大学学报2024，Vol.59Issue(2)：357-362,6.DOI:10.19405/j.cnki.issn1000-1492.2024.02.028
GNB4和Riplet基因甲基化检测在原发性肝癌诊断中的价值研究
The value of GNB4 and Riplet gene methylation detection in the diagnosis of primary liver cancer
摘要
Abstract
Objective To investigate the diagnostic efficacy and clinical value of GNB4 and Riplet gene methylation alone and in combination in the diagnosis of primary liver cancer.Methods A total of 313 patients were selected,including 78 patients with primary liver cancer,41 patients with other digestive system tumors,17 patients with non-digestive system tumors,20 patients with postoperative liver cancer,and 157 patients with benign liver disea-ses.The levels of GNB4 and Riplet gene methylation in plasma were detected using quantitative methylation-specific PCR(qMSP).Serum alpha-fetoprotein(AFP)levels were measured by direct chemiluminescence.Results The sensitivity and specificity of AFP in diagnosis were 51.3％and 94.3％,respectively;the sensitivity and specificity of GNB4 gene methylation in diagnosis were 83.3％and 99.4％,respectively;the sensitivity and specificity of Riplet gene methylation in diagnosis were 73.1％and 99.4％,respectively.The sensitivity and specificity of GNB4 and Riplet gene methylation combined diagnosis were 92.3％and 98.7％,respectively;the sensitivity and specificity of AFP,GNB4 and Riplet gene methylation combined diagnosis were 92.3％and 98.7％,respectively;the sensitivity and specificity of combined diagnosis including age and gender were 93.6％and 97.5％,respective-ly.Conclusion The sensitivity and specificity of AFP in the diagnosis of primary liver cancer are limited,while the methylation levels of GNB4 and Riplet genes are higher,and the sensitivity and specificity of their combined de-tection are higher than those of AFP.The sensitivity and specificity of AFP,GNB4 and Riplet gene methylation combined diagnosis are significantly higher than those of AFP,GNB4 and Riplet gene methylation alone.关键词
原发性肝癌/甲胎蛋白/基因甲基化/GNB4基因/Riplet基因Key words
primary liver cancer/alpha-fetoprotein/gene methylation/GNB4 gene/Riplet gene分类
医药卫生引用本文复制引用
杨玉萍,徐恩君,汪轩轩,唐宜桂,郑美娟,王悦,储梦真,徐家丹,王中新..GNB4和Riplet基因甲基化检测在原发性肝癌诊断中的价值研究[J].安徽医科大学学报,2024,59(2):357-362,6.基金项目
安徽省高校自然科学研究项目(编号:2023AH020048) （编号:2023AH020048）
