南方医科大学学报2024，Vol.44Issue(3)：594-604,11.DOI:10.12122/j.issn.1673-4254.2024.03.22
连翘配方颗粒与饮片的抗炎、抗肿瘤和抑菌效果的比较研究
Single extract of Forsythia Suspense versus the prepared drug in pieces:comparison of their anti-inflammatory,antitumor and antibacterial effects in zebrafish
摘要
Abstract
Objective To compare the anti-inflammatory,antitumor and anti-bacterial effects of the single extract(in granules)and the prepared drug in pieces of Forsythia Suspense(Lianqiao,a traditional Chinese herbal medicine).Methods In zebrafish embryo models of CuSO4 exposure,tail transection and LPS microinjection-induced inflammation,the anti-inflammatory effects of 10 μg/mL DEX,single extract of Forsythia Suspense,and the water extract of the prepared drug(400,600,and 800 μg/mL)were evaluated by observing neutrophil counts,RT-qPCR,HE staining and survival analysis.Zebrafish embryo models bearing different human tumor cell xenografts were used to assess the anti-tumor effect of the drugs in different dosage forms by fluorescence staining and HE staining.The microbroth dilution method was used to evaluate the antibacterial efficacy of the drugs.Results In the zebrafish embryo models of inflammation,both of the two dosage forms of Forsythia Suspense significantly inhibited neutrophil aggregation,reduced the mRNA expressions of TNF-α,IL-6,P38,Jnk,Erk and P65,and increased the survival rate of zebrafish.They both showed obvious inhibitory effects against xenografts of different human cancer cells including colon cancer cells(HCT116),pancreas adenocarcinoma cells(PANC-1),lung cancer cells(A549),liver cancer cells(Hep3B)and cervical carcinoma cells(Hela)in zebrafish embryos,and exhibited strong anti-bacterial effects at the concentration of 15.63 mg/mL.Conclusion The two dosage forms of Forsythia Suspense have similar anti-inflammatory,antitumor and antibacterial effects,but their effects for inhibiting IL-6,P65,and Jnk mRNA expressions and HCT116 cell proliferation differ significantly at low doses in zebrafish.关键词
连翘/中药配方颗粒/药效等效性/斑马鱼Key words
Forsythia Suspensa/sing extract of traditional Chinese medicine/pharmacological equivalence/zebrafish引用本文复制引用
郭新邓,郭卓琳,孙冬梅,邹丽芳,区锦莹,余林中,卢子滨,曹惠慧,刘俊珊..连翘配方颗粒与饮片的抗炎、抗肿瘤和抑菌效果的比较研究[J].南方医科大学学报,2024,44(3):594-604,11.基金项目
广东省基础与应用基础研究基金(2022A1515220081) （2022A1515220081）
