南方医科大学学报2024，Vol.44Issue(3)：411-419,9.DOI:10.12122/j.issn.1673-4254.2024.03.01
束缚应激通过激活Rho/ROCK通路诱导大鼠杏仁核血脑屏障的损伤
Restraint stress induces blood-brain barrier injury in rat amygdala by activating the Rho/ROCK signaling pathway
摘要
Abstract
Objective To investigate the role of Rho/ROCK signaling pathway in mediating restraint stress-induced blood-brain barrier(BBB)injury in the amygdala of rats.Methods Sixty male SD rats were randomized equally into control group(with food and water deprivation for 6 h per day),restraint stress group(with restraint for 6 h per day),stress + fasudil treatment(administered by intraperitoneal injection at 1 mg/100 g 30 min before the 6-h restraint)group,and fasudil treatment alone group.The elevated plus-maze test was used to detect behavioral changes of the rats,serum corticosterone and S100B levels were determined with ELISA,and Evans Blue leakage in the brain tissue was examined to evaluate the changes in BBB permeability.The changes in expression levels of tight junction proteins in the amygdala were detected using immunofluorescence assay and Western blotting,and Rho/ROCK pathway activation was detected by Pull-down test and Western blotting.Ultrastructural changes of the cerebral microvascular endothelial cells were observed using transmission electron microscopy.Results Compared with those in the control group,the rats in restrain stress group and stress+fasudil group showed obvious anxiety-like behavior with significantly increased serum corticosterone level(P<0.001).Compared with those in the control group and stress+fasudil group,the rat models of restrain stress showed more obvious Evans Blue leakage and higher S100B expression(P<0.01)but lower expressions of tight junction proteins in the amygdala.Pull-down test and Western blotting confirmed that the expression levels of RhoA-GTP,ROCK2 and P-MLC 2 were significantly higher in stress group than in the control group and stress + fasudil group(P<0.05).Transmission electron microscopy revealed obvious ultrastructural changes in the cerebral microvascular endothelial cells in the rat models of restrain stress.Conclusion Restraint stress induces BBB injury in the amygdala of rats by activating the Rho/ROCK signaling pathway.关键词
束缚应激/血脑屏障/杏仁核/Rho/ROCK通路/紧密连接/渗透性Key words
restraint stress/blood-brain barrier/amygdala/Rho/ROCK signaling pathway/tight junction/permeability引用本文复制引用
许光明,高安迪,丛斌..束缚应激通过激活Rho/ROCK通路诱导大鼠杏仁核血脑屏障的损伤[J].南方医科大学学报,2024,44(3):411-419,9.基金项目
中央司法警官学院博士科研启动经费专项项目(BSQDW202105) （BSQDW202105）
中央司法警官学院监狱学学院青年教研团队资助项目 （）
国家自然科学基金重点项目(81430047)Supported by National Natural Science Foundation of China(81430047). （81430047）
