南方医科大学学报2024，Vol.44Issue(3)：541-552,12.DOI:10.12122/j.issn.1673-4254.2024.03.16
高表达LINC00626通过JAK1/STAT3/KHSRP信号轴促进食管胃结合部腺癌转移的恶性进展
High LINC00626 expression promotes esophagogastric junction adenocarcinoma metastasis:the mediating role of the JAK1/STAT3/KHSRP axis
摘要
Abstract
Objective To investigate the role of JAK1/STAT3/KHSRP axis in mediating the regulatory effect of LINC00626 on progression of esophagogastric junction adenocarcinoma.Methods We collected surgical tumor and adjacent tissue specimens from 64 patients with esophagogastric junction adenocarcinoma and examined the expression levels of LINC00626 and KHSRP.qRT-PCR was used to detect the expressions of LINC00626 and KHSRP in 6 esophageal adenocarcinoma cell lines(OE-19,TE-7,Bic-1,Flo-1,SK-GT-4,and BE-3)and a normal esophageal epithelial cell line(HET-1A).OE-19 and TE-7 cell lines with stable LINC00626 knockdown and FLO-1 and SK-GT-4 cells stably overexpressing LINC00626 were constructed by lentiviral transfection,and the changes in proliferation,migration and invasion of the cells were evaluated using Cell Counting Kit-8(CCK-8)assay and Transwell migration/invasion assay.The expressions of KHSRP and JAK/STAT pathway proteins in the transfected cells were detected with Western blotting.The effects of LINC006266 knockdown and overexpression on subcutaneous tumor formation and lung metastasis of OE-19 and FLO-1 cell xenografts were tested in nude mice.Results The expression levels of LINC00626 and KHSRP were significantly increased in esophagogastric junction adenocarcinoma tissues and in esophageal adenocarcinoma cells.LINC00626 knockdown obviously inhibited the proliferation,migration and invasion of esophageal adenocarcinoma cells in vitro and decreased their tumor formation and lung metastasis abilities in nude mice,while overexpression of LINC00626 produced the opposite effects.In esophageal adenocarcinoma cells,LINC0626 knockdown significantly decreased and LINC00626 overexpression strongly enhanced the phosphorylation of JAK1 and STAT3.Conclusion High LINC00626 expression promotes esophageal-gastric junction adenocarcinoma metastasis by activating the JAK1/STAT3/KHSRP signal axis.关键词
食管胃结合部腺癌/LINC00626/JAK1/STAT3/KHSRP/转移Key words
esophagogastric junction adenocarcinoma/LINC00626/JAK1/STAT3/KHSRP/metastasis引用本文复制引用
张锋,范林林,康霞,韦海涛,李丽..高表达LINC00626通过JAK1/STAT3/KHSRP信号轴促进食管胃结合部腺癌转移的恶性进展[J].南方医科大学学报,2024,44(3):541-552,12.基金项目
河南省科技厅指导项目(242102310184) （242102310184）
河南省科技厅指导项目(242102310280) （242102310280）
河南省科技厅指导项目(242102310100):河南省科技厅指导项目(242102310197) （242102310100）
河南省教育厅资助项目(24A320002) （24A320002）
