结核与肺部疾病杂志2024，Vol.5Issue(2)：128-134,7.DOI:10.19983/j.issn.2096-8493.2024018
基于基因分型探讨北京市结核病患者复发原因及菌株遗传特征
Exploring the causes of recurrence and genetic characteristics of tuberculosis strains in Beijing based on genotypic analysis
摘要
Abstract
Objective:To investigate the causes of recurrence in tuberculosis patients in Beijing and the genetic characteristics of their strains,aiming to provide a scientific basis for the effective prevention and treatment of recurrent tuberculosis patients.Methods:The RD105 gene deletion method and 15-locus variable number tandem repeat genotyping experiment(VNTR)were used to compare the genotypes of Mycobacterium tuberculosis(MTB)of the initial and recurrent episodes in pulmonary tuberculosis patients in Beijing from January 2017 to December 2019,to elucidate the etiology of recurrent tuberculosis.The final isolates from recurrent patients were selected to evaluate the discriminatory ability of the genotyping technology on MTB strains of Beijing.Furthermore,a phylogenetic analysis was conducted to investigate the genetic characteristics of MTB prevalent in recurrent patients,providing insights into the evolutionary relationships within the strains associated with recurrent tuberculosis cases in Beijing.Results:The proportion of endogenous reactivation among recurrent patients was 65.4％(51/78),while the proportion of exogenous reinfection was 34.6％(27/78).Among the 78 MTB isolates obtained from recurrent patients,71 were Beijing genotype,and 7 were non-Beijing genotype.78 strains exhibited 68 distinct genotypes.Within 2 clonal complex clusters,13 isolates formed 4 clusters,while among 24 unique strains,2 isolates formed 1 cluster,resulting in a clustering rate of 12.8％.The hunter-gaston discrimination index for all MTB strains across the 15 VNTR loci was 0.994.Conclusion:The primary cause of recurrent tuberculosis in Beijing was endogenous reactivation,with a relatively low clustering rate.The predominant strain was Beijing genotype,exhibiting a high degree of genetic diversity.关键词
复发/分枝杆菌,结核/基因型Key words
Recurrence/Mycobacterium tuberculosis/Genotype分类
临床医学引用本文复制引用
张洁,丁北川,任怡宣,田丽丽,易俊莉,庞梦迪,杨新宇..基于基因分型探讨北京市结核病患者复发原因及菌株遗传特征[J].结核与肺部疾病杂志,2024,5(2):128-134,7.基金项目
Capital's Fund for Health Impovement and Research(2024-2G-30114) （2024-2G-30114）
Research and Cultivation Project of Beijing Center for Disease Control and Prevention(2023-KYJH-05 （）
2023-KYJH-07)首都卫生发展科研专项(2024-2G-30114) （2024-2G-30114）
北京市疾 病预防控制中心科研培育专项(2023-KYJH-05 （）
2023-KYJH-07) （）
