扬州大学学报（农业与生命科学版）2024，Vol.45Issue(1)：59-66,8.DOI:10.16872/j.cnki.1671-4652.2024.01.008
基于16S rRNA研究铜污染对土壤细菌群落、抗生素抗性基因及基因转移的影响
A 16S rRNA-based study of the effect of copper contamination on antibiotic resistance genes and horizontal gene transfer in soil bacterial communities
摘要
Abstract
In this study,soil samples were collected from three planting ponds treated with 0,200 and 400 mg Cu2+,respectively,and all samples were sequenced by 16S rRNA high-throughput sequencing.Sequencing results showed that Cu contamination changed the bacterial community structure in soil,and the abundance of antibiotic resistance genes(ARGs)and horizontal gene transfer(HGT)related genes increased with the increase of Cu2+concentration.The resul-ting antibiotic resistance genes were mostly multidrug resistance genes.This study showed that under the stress of Cu contamination,the bacterial community structure in soil was changed,and the HGT between bacteria was promoted,while the ARGs were enriched.关键词
铜污染/细菌群落结构/抗生素抗性基因/基因水平转移Key words
copper contamination/bacterial community structure/antibiotic resistance genes/horizontal gene transfer分类
农业科技引用本文复制引用
杨潇,张娅,王宏归..基于16S rRNA研究铜污染对土壤细菌群落、抗生素抗性基因及基因转移的影响[J].扬州大学学报（农业与生命科学版）,2024,45(1):59-66,8.基金项目
国家自然科学基金资助项目(22172140) （22172140）
江苏省自然科学基金资助项目(BK20211328) （BK20211328）
