扬州大学学报（农业与生命科学版）2024，Vol.45Issue(1)：75-83,138,10.DOI:10.16872/j.cnki.1671-4652.2024.01.010
南蛇藤总萜抑制中性粒细胞胞外诱捕网形成的作用与机制研究
The effect and mechanism of the total terpenoids of Celastrus orbiculatus Thunb.on inhibiting the formation of neutrophil extracellular traps
摘要
Abstract
To investigate the mechanisms and the effect of the total terpenoids of Celastrus orbiculatus Thunb.(TTC)on NETs,the cell model of NETs formation induced by phorbol 12-myristate 13-acetate(PMA)was established by isola-ting and preparing neutrophils.The effects of TTC on the formation of NETs and their biological markers were studied by Wright-Giemsa Staining,scanning electron microscope and immunofluorescence.The Annexin V/PI experiment was used to study TTC on neutrophil apoptosis.Results showed that TTC could dose-dependently inhibit the formation of NETs induced by PMA;TTC(80 μg·mL-1)could block the release of neutrophil elastase(NE)and disrupt the formation of Nets associated DNANE complexes.TTC(80 μg·mL-1)significantly inhibited PMA-induced CitH3 expression;TTC can promote neutrophil apoptosis in a dose-dependent manner.This study suggests that TTC can inhibit the formation of neutrophil NETs,and its mechanism may be related to its direct induction of neutrophil apoptosis.关键词
南蛇藤总萜/中性粒细胞胞外诱捕网/凋亡/中性粒细胞弹性蛋白酶/瓜氨酸化组蛋白H3Key words
Celastrus orbiculatus Thunb./neutrophil extracellular traps/apoptosis/neutrophil elastase/citrullinated histone H3分类
农业科技引用本文复制引用
郭雅洁,朱淼,刘延庆,陶丽..南蛇藤总萜抑制中性粒细胞胞外诱捕网形成的作用与机制研究[J].扬州大学学报（农业与生命科学版）,2024,45(1):75-83,138,10.基金项目
国家自然科学基金资助项目(81803782) （81803782）
