扬州大学学报（农业与生命科学版）2024，Vol.45Issue(1)：84-92,9.DOI:10.16872/j.cnki.1671-4652.2024.01.011
青蒿素-丙酮层共组装纳米药制备、表征及体外抗肿瘤初步评价
Preparation,characterization and preliminary in vitro antitumor evaluation of artemisinin-acetone layer co-assembled nanomedicines
摘要
Abstract
To solve the problem of poor water solubility of artemisinin and its acetone layer(industrial extraction waste of artemisinin with polymethoxyflavonoids as the main component)which leads to the limitation of its antitumor applica-tion,the supramolecular co-assembled nanomedicine of artemisinin and acetone layer with homogeneous quality and stable structure was constructed by using the method of emulsification-solution volatilisation.Laser particle size apparatus,fou-rier transform infrared spectrometer and scanning electron microscope were applied to characterize them,and MTT and scratch assay were used to determine the effects of nanomedicines on the proliferation and migration ability of mouse-de-rived CT26 colon cancer cells.The results showed that the average particle size of the prepared nanomedicine was 106.93 nm,the drug loading capacity was 7.78％,and the encapsulation rate was 72％;the IC50 of the co-assembled drug(artemisinin∶acetone layer=1∶2)was artemisinin∶acetone layer=17.13 mg·L-1∶34.27 mg·L-1,which was significantly better than that of the single-use group of the drugs,and the scratching experiment demonstrated that the co-assembled drug had a significant inhibition effect on the migration of the tumor cells.In conclusion,the supramolecular co-assembled nanomedicine of artemisinin-acetone layer prepared in this study has strong anti-tumor activity,which lays an important foundation for the development of anti-tumor drugs and the recycling of traditional Chinese medicine extract waste.关键词
青蒿素/丙酮层/共组装/纳米药/抗肿瘤Key words
artemisinin/acetone layer/co-assembly/nanopharmaceutical/antitumor分类
农业科技引用本文复制引用
李宛俞,徐湘迪,薛昊,金旭东,许金晶,陈靖..青蒿素-丙酮层共组装纳米药制备、表征及体外抗肿瘤初步评价[J].扬州大学学报（农业与生命科学版）,2024,45(1):84-92,9.基金项目
国家自然科学基金资助项目(81760377) （81760377）
扬州大学大学生学术科技创新基金项目(X20220754) （X20220754）
