军事医学2024，Vol.48Issue(2)：88-94,7.DOI:10.7644/j.issn.1674-9960.2024.02.002
辐射损伤早期小鼠呼出气特征性挥发性有机化合物分析及诊断价值
Identification and diagnostic value of characteristic volatile organic compounds in exhaled breath of mice with early stage radiation injury
肖炳坤 1缪潇瑶 1李志恒 1郑晓飞 1黄荣清1
作者信息
- 1. 军事科学院军事医学研究院辐射医学研究所,北京 100850
- 折叠
摘要
Abstract
Objective To investigate the characteristic volatile organic compounds(VOCs)in exhaled breath and their diagnostic value in mice with early stage radiation injury.Methods The thermal desorption gas chromatography-mass spectrometry(TD-GC/MS)technique was used to analyze VOCs in exhaled breath of irradiated mice by 60Coγ-ray with 800 cGy.The characteristic VOCs in the early stage of radiation injury were identified,and a diagnostic model was established.Results The 30-day survival rate of mice was 4.2%.There were significant differences in characteristic VOCs at 7 hours after radiation injury,and thirty characteristic VOCs related to early-stage radiation injury were identified.The diagnostic value of differential metabolites in mice after irradiation was evaluated via the ROC curve,and the area under the ROC curve(AUC)of a single compound exceeded 0.8.The diagnostic model was constructed by screening 9 potential biomarkers of exhalation through Fisher discriminant analysis,and its sensitivity and specificity were close to 100%.Conclusion Analysis of VOCs in exhaled breath is expected to provide a non-invasive diagnostic method for early screening and diagnosis of radiation injury.关键词
挥发性有机化合物/辐射损伤/呼出气/热脱附-气质联用/诊断模型Key words
volatile organic compounds/radiation injury/exhaled breath/thermal desorption gas chromatography-mass spectrometry/diagnostic model分类
医药卫生引用本文复制引用
肖炳坤,缪潇瑶,李志恒,郑晓飞,黄荣清..辐射损伤早期小鼠呼出气特征性挥发性有机化合物分析及诊断价值[J].军事医学,2024,48(2):88-94,7.
