军事医学2024，Vol.48Issue(2)：101-107,7.DOI:10.7644/j.issn.1674-9960.2024.02.004
高毒力且高耐药铜绿假单胞菌感染小鼠肺炎模型的构建与特征分析
Construction and characterization of a mouse model of pneumonia caused by highly virulent and multi-drug resistant Pseudomonas aeruginosa
摘要
Abstract
Objective To establish an inhalation infection pneumonia model of C57BL/6J mice with highly virulent and multi-drug resistant Pseudomonas aeruginosa(PA)strain F291007,and to study the microbiological,pathological and immunological characteristics of this model.Methods The strain F291007 was isolated and identified before the bacterial suspension was administered to the mice via aerosolized intratracheal inoculation to establish the pneumonia infection model.In the course of infection,the conditions and survival of the mice were observed,and the bacterial loads,the histopathological states and the cytokine expression levels in the major organs were detected.Finally,three key cytokines were blocked to observe the survival of mice.Results The strain F291007 was isolated and identified.After lethal dose infection,all the mice died within 24 h.After sub-lethal dose infection,a large number of immune cells in the body were capable of phagocytosis and killing of invading pathogens,which was manifested as rapid clearance of bacteria in lungs and the exponential decrease of bacterial load with the passage of time.The pathological changes in lungs were most severe at 1 to 3 days but gradually recovered.After infection,interleukin-6(IL-6),IL-17A and tumor necrosis factor-α(TNF-α)in alveolar lavage fluid and serum were significantly increased at 1 to 3 days.After blocking of these three cytokines with specific antibodies,the survival rates of infected mice decreased significantly.Conclusion A mouse model of gradually-recovered pneumonia infection caused by PA inhalation has been established,suggesting that the first one to three days are critical to immune response after infection through multiple indicators.This mouse model can be used for research on the pathogenesis,immunoregulation and treatment evaluation of highly virulent and multi-drug resistant PA inhalation pneumonia infection.关键词
铜绿假单胞菌/肺部感染/气溶胶/小鼠模型Key words
Pseudomonas aeruginosa/pulmonary infection/aerosol/mouse model分类
医药卫生引用本文复制引用
王林,张再青,陈方舟,吴妮尔,周冬生,胡凌飞..高毒力且高耐药铜绿假单胞菌感染小鼠肺炎模型的构建与特征分析[J].军事医学,2024,48(2):101-107,7.基金项目
国家重点研发计划(2022YFC2603900) （2022YFC2603900）
