聊城大学学报（自然科学版）2024，Vol.37Issue(2)：89-100,12.DOI:10.19728/j.issn1672-6634.2023120011
一株3,5,6-三氯-2-吡啶酚降解菌Delftia sp.DFT的分离鉴定及降解途径研究
Isolation,Identification and Degradation Pathway Study of a 3,5,6-trichloro-2-pyridinol Degrading Strain Delftia sp. DFT
摘要
Abstract
Microbial degradation is an effective method to eliminate 3,5,6-trichloro-2-pyridinol(TCP).However,there is still no detailed information on the mirobial metabolic mechanism of TCP.In order to explore the complete degradation pathway and metabolic mechanism of TCP.A TCP degrading strain was isolated from farmland soil.The degradation ability and the metabolites of TCP was detected by high per-formance liquid chromatography(HPLC)and liquid chromatography-mass spectrometry(LC-MS),re-spectively.The TCP degrading strain was identified as Delftia sp.DFT which could degrade 50 mg/L of TCP at a rate of 34.28％within 48 hours.This strain could also degrade other chloropyridine compounds such as 5-dichloro-2-pyridinol,6-chloro-2-pyridinol and 2-hydroxypyridine.Twelve TCP intermediate me-tabolites were detected in strain DFT and two possible degradation pathways of TCP were proposed on the basis of LC-MS analysis.The hydrolyzation-oxidation dechlorination pathway,the reduction dechlorina-tion pathway,the denitrification pathway and the alkylation pathway might be involved in TCP biodegra-dation by strain DFT.This is the first report on four different pathways responsible for TCP degradation in the genus Delftia sp.,which provides new information for the study of TCP degradation mechanism in pure culture.关键词
3,5,6-三氯-2-吡啶酚/降解菌/分离鉴定/代尔夫特菌/降解途径Key words
3,5,6-trichloro-2-pyridinol/degrading bacteria/isolation and identification/Delftia sp./deg-radation pathway分类
资源环境引用本文复制引用
贾楠,岳彩旭,吕雪茹,王雪婷,丁宁,王圣惠..一株3,5,6-三氯-2-吡啶酚降解菌Delftia sp.DFT的分离鉴定及降解途径研究[J].聊城大学学报（自然科学版）,2024,37(2):89-100,12.基金项目
山东省自然科学基金面上项目(ZR2020MD103) （ZR2020MD103）
国家自然科学基金青年基金项目(41401288) （41401288）
聊城大学畜牧学学科开放课题(319312101-19) （319312101-19）
大学生创新创业训练项目(CXCY2022055,CXCY2023335,CXCY2023339)资助 （CXCY2022055,CXCY2023335,CXCY2023339）
