聊城大学学报（自然科学版）2024，Vol.37Issue(2)：101-110,10.DOI:10.19728/j.issn1672-6634.2023110012
生物法合成熊去氧胆酸研究进展
Bio-Synthesis Progress of Ursodeoxycholic Acid
摘要
Abstract
Ursodeoxycholic acid(UDCA)is a fundamental medication in the treatment of hepatobiliary dis-eases and used as an adjuvant in the treatment of certain cancers and neurological disorders.The chemical synthesis method of UDCA is environmentally unfriendly,low yields output and cannot be fully met the production demand yet.In contrast,the biosynthesis of UDCA is characterized by high conversion rates,low energy conservation,and environmental friendliness.At present,it represent the direction of research and development for UDCA synthesis.Availabled substrates are utilized for biosynthesis such as chenode-oxycholic acid(CDCA),cholic acid(CA),or lithocholic acid(LCA).substrates are catalyzed by four en-zymes:7alpha-hydroxysteroid dehydrogenase(7a-HSDH),7beta-hydroxysteroid dehydrogenase(7β-HS-DH),Lactate dehydrogenase(LDH),and Glucose dehydrogenase(GDH).There are two methods for syn-thesizing UDCA.It includes free enzyme catalysis and whole-cell synthesis.Free enzyme catalysis method are subdivided into"one-pot two-step"and"one-pot one-step".Whole-cell catalysis uses engineered cells as reaction vessels.The synthesis of UDCA is catalyzed by enzyme systems of cell.This paper reviews This article gives an overview of the enzymes involved,synthesis methods and the progress in synthesis technology for both free enzyme catalysis and whole-cell synthesis of UDCA.It provides technical refer-ences for the research and production of UDCA.关键词
熊去氧胆酸/生物法合成/游离酶催化合成/全细胞合成Key words
ursodeoxycholic acid/biological synthesis/free enzyme-catalyzed synthesis/whole-cell syn-thesis分类
医药卫生引用本文复制引用
张雪,张悦,刘梦雨,栾浩妮,宋鹏,周正松,王飞,徐伟..生物法合成熊去氧胆酸研究进展[J].聊城大学学报（自然科学版）,2024,37(2):101-110,10.基金项目
国家自然科学基金青年项目(82304838) （82304838）
山东省自然科学基金面上项目(ZR2022MC159)资助 （ZR2022MC159）
