军事医学2024，Vol.48Issue(2)：115-121,7.DOI:10.7644/j.issn.1674-9960.2024.02.006
基于量子点标记胶质纤维酸性蛋白的轻度颅脑损伤快速诊断试纸条研制及评价
Development and evaluation of mild TBI rapid diagnostic strips based on GFAP labeled with quantum dots
俞昶乐 1付文亮 1邢微微 1徐东刚1
作者信息
- 1. 军事科学院军事医学研究院军事认知与脑科学研究所,北京 100850
- 折叠
摘要
Abstract
Objective To establish an auxiliary method for diagnosis of mild traumatic brain injury based on serum GFAP rapid detection test strips using immunochromatographic technology labeled with quantum dot microspheres.Methods The quantum dot microspheres were coupled with GFAP antibodies.The detection conditions were optimized to obtain the fluorescence probe in order to prepare the immunochromatographic test strips.An auxiliary diagnostic method was established after optimization of detection conditions.Finally,the auxiliary diagnostic effect of the test strips was evaluated using clinical samples.Results The serum concentration of GFAP could be detected by the optimized test strips within 13 mins with a detection limit of 0.15 ng/mL,and no more than 70µL of the serum sample was required.In addition,good reproducibility was achieved by different batches of test strips(CV=10.7%).The detection sensitivity and specificity of the strips to mild traumatic brain injury using 51 clinical samples were 95.24%and 96.67%respectively,indicating good effects of detection.Conclusion The developed test strips are user-friendly with reliable results,which can facilitate field rapid diagnosis of mild traumatic brain injury in complicated wartime environments.关键词
轻度颅脑损伤/量子点/胶质纤维酸性蛋白/免疫层析试纸条/快速诊断Key words
mild traumatic brain injury/quantum dot/glial fibrillary acid protein/immunochromatographic test strip/rapid diagnosis分类
医药卫生引用本文复制引用
俞昶乐,付文亮,邢微微,徐东刚..基于量子点标记胶质纤维酸性蛋白的轻度颅脑损伤快速诊断试纸条研制及评价[J].军事医学,2024,48(2):115-121,7.
