南京林业大学学报（自然科学版）2024，Vol.48Issue(2)：121-128,8.DOI:10.12302/j.issn.1000-2006.202305030
H2S供体NaHS对渗透胁迫下美国剑麻干旱适应性及抗氧化特性的影响
Effects of H2S donor NaHS on the adaptability and antioxidant properties of Agave americana plantlets under an in vitro culture of osmotic stress
摘要
Abstract
[Objective]Agave americana is an important crassulacean acid metabolism(CAM)plant with high economic value in tropical areas.Studying its drought adaptability could provide evidence of the drought tolerance mechanism,and support for resource development and engineering research regarding the utilization of CAM plants.[Method]In this study,A.americana plantlets were treated with 1.0 mmol/L NaHS(NS),10％polyethylene glycol(PEG10),20％PEG(PEG20),1.0 mmol/L NaHS+10％PEG(NS+PEG10),and 1.0 mmol/L NaHS+20％PEG(NS+PEG20)under an in vitro culture.The responses to PEG osmotic stress were studied and the effects of H2S donor NaHS on osmotic regulation and antioxidant properties in A.americana were investigated.[Result]The results showed that the A.americana plantlets could survive under the 20％PEG(high concentration)treatment and had a degree of drought resistance.However,injury symptoms and oxidative stress reactions occurred,with the fresh mass decreasing by 16.6％in PEG20.The cell ultrastructure changed,and the malondialdehyde(MDA)and H2O2 contents increased in PEG20.Despite this,the plantlets regulated osmotic pressure and reduced the stress intensity by increasing the levels of proline and soluble sugars.In the presence of PEG,H2S donor NaHS could reduce the excessive accumulation of proline and H2O2.Furthermore,H2S could activate superoxide dismutase(SOD)and five antioxidases,and increased the glutathione(GSH)content to clear active oxygen species(ROS)and active carbonyl compounds,subsequently enhancing the antioxidant capacity.[Conclusion]The A.americana plantlets had a certain degree of drought tolerance.The H2S had an important role in osmotic regulation and regulating antioxidant levels,making the plantlets better adapted to drought conditions.关键词
美国剑麻/渗透胁迫/硫化氢/抗旱性/渗透调节/抗氧化作用Key words
Agave americana/osmotic stress/H2S/drought resistance/osmotic regulation/oxidation resistance分类
农业科技引用本文复制引用
沈瑒,狄晶晶,陈颖,冯凯,陆锦玲,胡宇辰..H2S供体NaHS对渗透胁迫下美国剑麻干旱适应性及抗氧化特性的影响[J].南京林业大学学报（自然科学版）,2024,48(2):121-128,8.基金项目
国家重点研发计划(2017YFD0601301) （2017YFD0601301）
江苏高校优势学科建设工程资助项目(PAPD). （PAPD）
