西南医科大学学报2024，Vol.47Issue(2)：123-129,7.DOI:10.3969/j.issn.2096-3351.2024.02.006
丁酸前药BBA纳米晶的构建及其对结肠癌细胞的抑制作用
Construction of BBA nanocrystals and its inhibitory effect on colon cancer cells
摘要
Abstract
Objective In this study,2-butyloxide-5-butylaminobenzoic acid(BBA)was synthesized with butyric acid and 5-aminosalicylic acid,which can be used as the prodrug for the treatment of colon cancer.The sustained-release preparation of BBA nano-crystals expected with good targeting and high bioavailability was prepared by using nanotechnology,which could effectively solve the problems of antitumor drug solubility and pharmaceutical properties.Methods BBA nanocrystals were prepared by solvent-antisolvent precipitation,and were characterized by NMR scanning,infrared spectrometer and transmission electron microscopy.High-performance liquid chromatography(HPLC)was used to detect the content of BBA.The cell viability of SW620 cells treated with different concen-trations of BBA,BBA nanocrystals,blank nanocrystals,5-fluorouracil(5-FU),5-aminosalicylic acid(5-ASA)and sodium butyrate(NaB)were detected by MTT assay.Results BBA nanocrystals were successfully prepared.MTT results showed that the in vitro inhibi-tory effect of BBA nanocrystals on colon cancer cells of SW620 was slightly lower than that of the positive control group of 5-FU(P>0.05),but was similar to the other groups at the same concentration,indicating that BBA nanocrystals showed desire inhibitory effect on colon cancer cells SW620 in vitro(P<0.05).Conclusion In the present study,we successfully prepared the nanocrystals of BBA as the pro-drug of butyric,which proved that the new drug preparation showed an inhibitory effect on colon cancer cells,and might provide an extremely significant new idea and original approach for the clinical treatment of colon cancer.关键词
结肠癌/丁酸/5-氨基水杨酸/纳米晶Key words
Colon cancer/Butyric acid/5-Aminosalicylic acid/Nanocrystals分类
医药卫生引用本文复制引用
林燕,钟志容,兰华琪,吕诺男,熊梦佳,王诚梽,吴越,孙小端,杜兴杰,刘中兵..丁酸前药BBA纳米晶的构建及其对结肠癌细胞的抑制作用[J].西南医科大学学报,2024,47(2):123-129,7.基金项目
四川省科技厅项目(2022YFS0627,2022YFS0188) （2022YFS0627,2022YFS0188）
泸州市科技计划项目(2023JYJ023) （2023JYJ023）
大学生创新创业训练计划(202310632075,202210632003) （202310632075,202210632003）
教育部'春晖计划'合作科研项目(202200618) （202200618）
