少花蒺藜草荧光定量PCR内参基因筛选与验证OA

Cenchrus pauciflorus Benth.is a genus of Tribulus terrestris in the family Poaceae,which is an annual malignant weed that invades northern China,and the invasion trend of Cenchrus pauciflorus Benth.is expanding year by year,so it is particularly important to study the gene of Cenchrus pauciflorus Benth.and the prevention and control of Cenchrus pauciflorus Benth.at the molecular level.In this study,based on the transcriptome data of Cenchrus pauciflorus Benth.,6 Internal reference genes were selected,the expression of Internal reference genes was detected by Real-time PCR,and the best reference genes were comprehensively evaluated and screened through five evaluation methods:geNorm,NormFinder,BestKeeper,comparative △Ct method and RefFinder.The results showed that the comprehensive ranking of the six candidate reference genes was TCTP1>RH37>ACX4>TCTP2>GAPDH>TBP,TCTP1 gene was the highest and most stable expression in different tissue samples of Cenchrus pauciflorus Benth.at different stages,and the most unstable expression was the gene TBP,TCTP1 was the best internal reference gene of Cenchrus pauciflorus Benth.under comprehensive evaluation,so TCTP1 could be used as an internal reference gene for the detection of Cenchrus pauciflorus Benth.Real-time PCR.This study provides a theoretical basis for the follow-up genetic study of Cenchrus pauciflorus Benth.and the biological control of Cenchrus pauciflorus Benth.at the molecular level.