现代农业研究2024，Vol.30Issue(3)：10-16,7.
少花蒺藜草荧光定量PCR内参基因筛选与验证
Screening and Verification of Internal Reference Gene of Cenchrus pauciflorus Benth.by Real-time PCR
摘要
Abstract
Cenchrus pauciflorus Benth.is a genus of Tribulus terrestris in the family Poaceae,which is an annual malignant weed that invades northern China,and the invasion trend of Cenchrus pauciflorus Benth.is expanding year by year,so it is particularly important to study the gene of Cenchrus pauciflorus Benth.and the prevention and control of Cenchrus pauciflorus Benth.at the molecular level.In this study,based on the transcriptome data of Cenchrus pauciflorus Benth.,6 Internal reference genes were selected,the expression of Internal reference genes was detected by Real-time PCR,and the best reference genes were comprehensively evaluated and screened through five evaluation methods:geNorm,NormFinder,BestKeeper,comparative △Ct method and RefFinder.The results showed that the comprehensive ranking of the six candidate reference genes was TCTP1>RH37>ACX4>TCTP2>GAPDH>TBP,TCTP1 gene was the highest and most stable expression in different tissue samples of Cenchrus pauciflorus Benth.at different stages,and the most unstable expression was the gene TBP,TCTP1 was the best internal reference gene of Cenchrus pauciflorus Benth.under comprehensive evaluation,so TCTP1 could be used as an internal reference gene for the detection of Cenchrus pauciflorus Benth.Real-time PCR.This study provides a theoretical basis for the follow-up genetic study of Cenchrus pauciflorus Benth.and the biological control of Cenchrus pauciflorus Benth.at the molecular level.关键词
少花蒺藜草/荧光定量PCR/内参基因Key words
Cenchrus pauciflorus Benth./real-time PCR/internal reference genes分类
农业科技引用本文复制引用
徐礼莹,陈宇杰,田迅,百灵,吴佳丽,张福霖,黄菊,马文静,吕广超,马红悦..少花蒺藜草荧光定量PCR内参基因筛选与验证[J].现代农业研究,2024,30(3):10-16,7.基金项目
国家自然基金项目"科尔沁草原外来入侵植物少花蒺藜草CpPRX1基因调控刺苞表皮毛木质化形态建成的分子机制研究"(项目编号:32260683) （项目编号:32260683）
国家自然基金项目"少花蒺藜草生活史策略与植物-土壤反馈互作关系调节干旱适应性的作用机制研究"(项目编号:32360267) （项目编号:32360267）
内蒙古自治区硕士研究生科学创新项目"少花蒺藜草刺苞表皮毛分支基因ANGUSTIFOLIA基因克隆及功能鉴定"(项目编号:S20231211Z) （项目编号:S20231211Z）
内蒙古自治区大学生创新创业训练项目"少花蒺藜草的遗传转化体系的构建"(项目编号:S202310136027). （项目编号:S202310136027）
