饲料研究2024，Vol.47Issue(5)：66-71,6.DOI:10.13557/j.cnki.issn1002-2813.2024.05.013
超声波辅助提取紫苏叶中总黄酮及其抗运动疲劳活性的研究
Study on ultrasonic assisted extraction of total flavonoids from Perilla frutescens leaves and its anti-exercise fatigue activity
摘要
Abstract
The response surface method was used to optimize the ultrasonic-assisted extraction of total flavonoids from Perilla frutescens leaves based on a single-factor test.The anti-fatigue activity of the total flavonoid extract from Perilla frutescens leaves was studied in mice.The results showed that the primary and secondary factors affecting the extraction rate of total flavonoids from Perilla frutescens leaves were:Ethanol concentration＞extraction temperature＞liquid-solid ratio＞extraction time.The optimal extraction conditions were ethanol concentration 85％,liquid-solid ratio 15 mL/g,extraction temperature 70 ℃,and extraction time 35 min.Under those conditions,the average extraction rate of total flavonoids from Perilla frutescens leaves was 4.67％,which was highly consistent with the predicted value of 4.61％,indicating the reliability of the method.It can be used for the extraction of total flavonoids from Perilla frutescens leaves with the practical value.Compared with the control group,the swimming time of mice bearing weights increased significantly after receiving the total flavonoid extract from Perilla frutescens leaves.The levels of blood lactic acid(BLA)and serum urea nitrogen(BUN)were significantly reduced compared with the control group(P＜0.05).It can be concluded that the total flavonoid extract of Perilla frutescens leaves has a good alleviating effect on the body's anti-fatigue.关键词
紫苏叶/超声波辅助提取/响应面法/黄酮/抗疲劳Key words
Perilla frutescens leaves/ultrasonic-assisted extraction/response surface method/flavonoids/anti-fatigue分类
农业科技引用本文复制引用
谢彬,张红静,冯志强,黄华生..超声波辅助提取紫苏叶中总黄酮及其抗运动疲劳活性的研究[J].饲料研究,2024,47(5):66-71,6.基金项目
河南省新乡市科技计划项目(项目编号:2022HN0786) （项目编号:2022HN0786）
