兰州大学学报（医学版）2024，Vol.50Issue(1)：7-15,9.DOI:10.13885/j.issn.1000-2812.2024.01.002
环泊酚可抑制System Xc-/GPX4通路介导的铁死亡减轻大鼠肾缺血再灌注损伤
Ciprofol can alleviate renal ischemia-reperfusion injury in rats via ferroptosis SystemXc-/GPX4 pathway
摘要
Abstract
Objective To investigate the protective effect of Ciprofol on renal ischemia-reperfusion injury and its mechanism.Methods The established renal ischemia-reperfusion model of SD rats was intervened by several drugs besides Ciprofol,ferrostatin-1(inhibitor of ferroptosis)and Erastin(agonist of ferroptosis)alone or in combination.After 24 hours,the rats were sacrificed under anesthesia,and their heart blood taken to detect creatinine and urea nitrogen to evaluate the renal function.At the same time,the kidneys of rats were taken,part of which stained with hematoxylin-eosin staining and the paller score was used to evaluate the degree of renal pathological damage in rats.The other part was used to detect the level of ferroptosis-related indicators of reactive oxygen species,glutathione,ferrous iron,malondialdehyde,the expression and tran-scription level of SLC7A11,the main subunit of SystemXc-,and the downstream functional protein GPX4.Results Compared with the control group,the levels of creatinine,urea nitrogen,paller score,reactive oxygen speciesferrous iron,malondialdehyde in the Ciprofol and Fer-1 group were lower(P<0.05).Compared with the control group,the levels of glutathione,SLC7A11,GPX4 in the Ciprofol group were higher;besides,the expression levels of SLC7A11 and GPX4 related genes were higher in the Ciprofol group(P<0.05).Com-pared with the Ciprofol group,the levels of glutathione,SLC7A11,GPX4 and the expression levels of SLC7A11 and GPX4 related genes were lower in the Ciprofol+Erastin group(P<0.05).Conclusion Ciprofol can reduce renal ischemia-reperfusion injury to protect the kidney,and the mechanism is related to the System-Xc-/GPX4 pathway.关键词
肾缺血再灌注损伤/铁死亡/胱氨酸/谷氨酸反向转运蛋白/谷胱甘肽过氧化物酶4通路/环泊酚Key words
ischemia-reperfusion injury/ferroptosis/systemXc-/GPX4 axis/Cipofol分类
医药卫生引用本文复制引用
罗豪,魏华锋,李灵玉,贺佳辉,姚雅韡,吕兴华..环泊酚可抑制System Xc-/GPX4通路介导的铁死亡减轻大鼠肾缺血再灌注损伤[J].兰州大学学报（医学版）,2024,50(1):7-15,9.基金项目
甘肃省自然科学基金资助项目(23JRRA0932) （23JRRA0932）
甘肃省重点人才资助项目(2021RCXM002) （2021RCXM002）
兰州大学第一医院院内基金资助项目(ldyyyn2020-106 （）
ldyyyn2022-1) （）
兰州大学医学教育创新发展资助项目(lzuyxcx-2022-130) （lzuyxcx-2022-130）
