饲料研究2024，Vol.47Issue(5)：72-75,4.DOI:10.13557/j.cnki.issn1002-2813.2024.05.014
饲用桑叶多糖提取工艺优化及其体外抗菌活性研究
Optimization of forage mulberry leaf polysaccharide extraction process and its in vitro antimicrobial activity
摘要
Abstract
In this experiment,polysaccharide was extracted from forage mulberry leaf by hot water extraction.To improve the extraction rate of polysaccharide in mulberry leaf,the effects of liquid-to-material ratio,extraction temperature,and extraction time on the polysaccharide extraction rate were studied by single factor experiment,combination of process parameters for the extraction of mulberry leaf polysaccharide was optimized by orthogonal analysis,and the antibacterial activity of mulberry leaf polysaccharide in vitro was detected by Oxford cup method.The results showed that the effects of various factors on the extraction rate of mulberry leaf polysaccharide were ranked as extraction temperature＞liquid-to-material ratio＞extraction time.The optimal combination of production process parameters was as follows:Liquid-material ratio of 30 L/g,extraction temperature of 90 ℃,and extraction time of 3 h.Under the optimal process conditions,the extraction rate of mulberry leaf polysaccharide was 6.69％.Mulberry leaf polysaccharide had obvious inhibitory effects on Staphylococcus aureus and Escherichia coli,with the diameter of the inhibition zone being 9.22 mm and 11.74 mm,respectively,and the lowest concentration of antibacterial was 2.0 g/L.The results showed that the mulberry leaf polysaccharide extracted by hot water extraction method could be used as functional feed additives in animal breeding.关键词
饲用桑叶/多糖/热水浸提法/抑菌活性Key words
forage mulberry leaf/polysaccharide/hot water extraction/antimicrobial activity分类
农业科技引用本文复制引用
尚朝利,白泽方,樊轻亚..饲用桑叶多糖提取工艺优化及其体外抗菌活性研究[J].饲料研究,2024,47(5):72-75,4.基金项目
中共河南省委教育工委高校"双带头人"教师党支部书记工作室建设项目 （）
