正己醇对机械伤香蕉采后生理特性影响及磷脂酶D的抑制作用OA北大核心CSTPCD

To maintain the storage quality and prolong the storage period of postharvest bananas,the present study used'Guijiao No.6'as the test material to investigate the 1-hexanol effect on the fruit quality and storability of the posthar-vest bananas under room temperature storage conditions.Banana fruits with intact fruit appearance and similar ripeness and size were selected and randomly divided into control and treatment groups.The control and treatment groups were treated with water and 0.1%(V/V)aqueous solution of 1-hexanol,respectively.Then,the bananas were removed to air-dry and packaged in polyethylene bags with a zipper.Finally,the samples were stored at 25℃for 15 days.Changes in firmness,soluble solids,respiration intensity,condition index,malondialdehyde(MDA),membrane permeability,and PLD activity were measured every 3 days,and changes in PLDγ and PLDζ expression during storage were analyzed by quantitative real-time PCR(qRT-PCR).The results showed that 0.1%(V/V)1-hexanol significantly(P<0.05)delayed the process of postharvest banana fruit softening,and the delaying effect was highly(P<0.01)significant on days 9 and 12.From day 9,1-hexanol highly significantly inhibited the rise in total soluble solids and condition index.From day 6,1-hexanol significantly inhibited respiration,where the peak respiration was reached on day 9 and was highly signifi-cantly reduced by 33.93%compared to the control.After day 12,compared with the control group,1-hexanol highly significantly reduced the rise of MDA content and membrane permeability,by 26.70%and 62.20%on day 15,respec-tively.In addition,1-hexanol significantly inhibited PLD activity,especially from 6 to 12 days,and the inhibition was highly significant(P<0.01).At day 15,compared with the control group,1-hexanol highly significantly reduced the banana expression of PLDγ and PLDζ by 72.06%and 33.08%,respectively.In conclusion,1-hexanol can effectively delay postharvest banana fruit softening,inhibit the respiration and PLD activity,and reduce postharvest fruit disease,thereby inhibiting total soluble solids,MDA production,and increased membrane permeability,and inhibiting PLDγ and PLDζ expression,thereby maintaining postharvest fruit storage quality and extending storage life.Therefore,1-Hexanol can be used as a fruit preservative,which is not only important for maintaining the post-harvest fruit quality and the development of the banana industry,but also has good application prospects during post-harvest storage.