饲料研究2024，Vol.47Issue(5)：104-108,5.DOI:10.13557/j.cnki.issn1002-2813.2024.05.020
基于HPLC检测市售博落回注射液中血根碱和白屈菜红碱的成分含量
To determine content of sanguinarine and chelerythrine in Macleaya cordata injection based on HPLC
摘要
Abstract
The purpose of the trial was to evaluate the quality of Macleaya cordata injection product.In the experiment,high performance liquid chromatography was used to establish a simultaneous method for the determination of sanguinarine and chelryerythrine in Macleaya cordata injection products.Agilent mAbylent mAb was used as acetonitrile-0.1％phosphoric acid for gradient elution at a flow rate of 1.0 mL/min,a column temperature of 30 ℃,a detection wavelength of 270 nm,and an injection volume of 5 μL.The results showed that the HPLC method for the determination of sanguinarine and chelerythrine in Macleaya cordata injection had high specificity,good linear range,and good repeatability.The precision RSDs of sanguinarine and ceracernine were 0.45％and 0.09％,the stability RSDs were 0.60％and 0.39％,respectively,and the recoveries ranged from 99.83％～101.79％and 103.47％～105.56％,respectively.The study indicates that the method is stable and reliable,with high precision and accuracy,and can be used to evaluate the quality of commercially available Macleaya cordata injection.关键词
博落回注射液/血根碱/白屈菜红碱/HPLC/质量评价Key words
Macleaya cordata injection/sanguinarine/chelerythrine/HPLC/quality evaluation分类
农业科技引用本文复制引用
陈思雅,张玮琛,邱文艳,刘晓轩,杨晶..基于HPLC检测市售博落回注射液中血根碱和白屈菜红碱的成分含量[J].饲料研究,2024,47(5):104-108,5.基金项目
湖南省自然科学基金项目(项目编号:NO.2023JJ60479) （项目编号:NO.2023JJ60479）
湖南省教育厅科学研究项目(项目编号:22B0373) （项目编号:22B0373）
湖南中医药大学中药学一流学科项目 （）
湖南中医药大学研究生创新项目(项目编号:2023CX155) （项目编号:2023CX155）
