饲料研究2024，Vol.47Issue(5)：109-116,8.DOI:10.13557/j.cnki.issn1002-2813.2024.05.021
分散固相萃取-超高效液相色谱-串联质谱法测定饲料中的多肽类抗生素
Determination of polypeptide antibiotics in feed by dispersive solid-phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A quantitative method for the simultaneous determination of 16 peptide antibiotics(PPTs)in feed was established based on dispersed solid-phase extraction and purification combined with ultra-performance liquid chromatography-tandem mass spectrometry.The samples were extracted by 1％trichloroacetic acid(aq)-methanol solution,and purified by dispersed solid-phase extraction of octadecyl silica gel(C18)before being measured on the machine.In electrospray positive ionization mode,multiple reaction monitoring(MRM)mode was used to quantify the matrix-matched correction curve.The results showed that the linear relationship of PPTs is good in the corresponding concentration range,the correlation coefficient(R2)was larger than 0.99,the detection limit was 0.4～12.1 μg/kg,and the quantification limit was 1.4～40.4 μg/kg.The average recovery rate(n=6)and relative standard deviation(RSD)of PPTs in feed were 80.0％～114.4％,and the relative standard deviation(RSD)was 2.33％～9.51％.The results show that the method is simple,accurate and reliable,with high sensitivity,short analysis time and high reproducibility,and can be widely used in the rapid screening and routine monitoring of 16 PPTs in feed.关键词
超高效液相色谱-串联质谱/多肽类抗生素/饲料/分散固相萃取Key words
ultra-performance liquid chromatography-tandem mass spectrometry/peptide antibiotics/feed/dispersive solid-phase extraction分类
农业科技引用本文复制引用
黄燕,李燕妹,陈言凯,梁敏,冯丽凤,陈何燊,林浩学,蔡小明..分散固相萃取-超高效液相色谱-串联质谱法测定饲料中的多肽类抗生素[J].饲料研究,2024,47(5):109-116,8.基金项目
福建省产品质量检验研究院科研项目(项目编号:KY202115A) （项目编号:KY202115A）
