热带作物学报2024，Vol.45Issue(3)：592-601,10.DOI:10.3969/j.issn.1000-2561.2024.03.016
槟榔籽提取物对血管紧张素转化酶(ACE)活性的抑制作用研究
Inhibitory Effects of Areca Nut Extract on the Activity of Angiotensin Converting Enzyme
摘要
Abstract
In this study,75%ethanol solution was used to prepare the total extracts of arecanut nut(ANE).Then differ-ent fractions of the extracts were prepared by fractionating with petroleum ether(PE-ANE),ethyl acetate(EAC-ANE),n-butanol(BU-ANE)and water(W-ANE)in turn.The inhibition of hypertension from ANE was determined by angio-tensin converting enzyme(ACE)activity inhibition assay in vitro.The inhibitory was evaluated with the fraction dis-playingthe best ACE inhibitory activity by the enzyme kinetic method.In addition,the contents of total polyphenols,total sugar and total protein in each fraction of ANE were determined.The correlation between the contents of the compositions and ACE inhibitory activities was analyzed.Finally,phenoliccomponents in the four fractions were ana-lyzed.The results showed that all the fractions of ANE at different polar parts exhibited ACE inhibitory activity,and EAC-ANE displayed the best.Enzyme kinetics tests showed that the inhibitory effect of EAC-ANE from tender areca on ACE was a mixed type of competitive inhibition and non-competitive inhibition.The correlation analysis showed that the IC50 value of the ACE inhibitory activity of each polar part of ANE was significantly negatively correlated with the content of polyphenols and total sugar.And the absolute value of the correlation coefficient between the IC50 value of ACE inhibitory activity and the polyphenol content reached a very strong correlation(r=-0.912).And a variety of polyphenol components such as catechin,L-epicatechin,procyanidin B2,protocatechuic acid,and quercetin were identi-fied in the four different polar parts,and the content of polyphenol components in EAC-ANE was the highest.In con-clusion,ANE has a certain blood pressure lowering potential,and the ethyl acetate extraction site is the best,and the polyphenol content has a strong correlation with the ACE inhibitory activity of each extract.It can be preliminarily in-ferred that polyphenols may be the main substances that inhibit the activity of ACE in ANE.关键词
槟榔提取物/血管紧张素转化酶/抑制作用/酶动力学Key words
areca nut extract/angiotensin converting enzyme(ACE)/inhibitory activity/enzyme kinetics分类
医药卫生引用本文复制引用
于鑫,唐敏敏,罗家欣,宋菲,朱婷婷,陈华,赵松林..槟榔籽提取物对血管紧张素转化酶(ACE)活性的抑制作用研究[J].热带作物学报,2024,45(3):592-601,10.基金项目
海南省自然科学基金项目(No.322RC791) （No.322RC791）
海南省重点研发计划项目(No.ZDYF2022XDNY205). （No.ZDYF2022XDNY205）
