热带作物学报2024，Vol.45Issue(3)：602-608,7.DOI:10.3969/j.issn.1000-2561.2024.03.017
无核荔枝僵果病的病原菌鉴定、入侵途径及快速检测技术
Identification,Invasion Pathway and Rapid Detection Technology of New Mummy Fruit Pathogen of Seedless Litchi
摘要
Abstract
In 2022,a stiff fruit disease was found on seedless litchi in Hainan,which was obviously different from the common litchi diseases in Hainan,such as anthracnose and acid rot.It mainly damaged young fruits.There was no sig-nificant change in the appearance of young fruits when the disease occurred.When the fruit was broad bean,the outer peel of the diseased fruit became darker,and the inner peel became irregularly brown after dissection.The fruit no longer expanded,forming fleshless stiff fruit,which was easy to cause serious economic losses.In this study,the pathogen of the disease was isolated,identified,invasion way and rapid detection techniques were studied.The patho-gen was identified as Fusarium pernambucanum by morphological identification,ITS,RPB2 and TEF1 gene sequence analysis.The pericarp of seedless litchi fruit at young fruit stage was susceptible to pathogen invasion after being dam-aged by farming operations,wind,piercing-sucking insects,while the fruit surface was not diseased without wounds.The main invasion period of the pathogen was 3.2-4.8 mm in the longitudinal direction of the fruit.After the fruit was bigger than 9.8 mm,the probability of pathogen invasion gradually decreased.Based on the TUB gene sequence,the specific primers for rapid detection of F.pernambucanum were obtained,and a rapid and efficient molecular detection method based on ordinary PCR was constructed.The detection method is sensitive and efficient,and would provide support for early diagnosis and prevention of seedless litchi stiff fruit disease.关键词
无核荔枝/僵果病/病原菌/鉴定/检测Key words
seedless litchi/fruit mummy/pathogen/identification/detection分类
农业科技引用本文复制引用
王伟博,李松刚,胡美姣,曹学仁,李敏,李焕苓,李芳,高兆银..无核荔枝僵果病的病原菌鉴定、入侵途径及快速检测技术[J].热带作物学报,2024,45(3):602-608,7.基金项目
国家重点研发计划项目(No.2020YFD10001003) （No.2020YFD10001003）
海南省自然科学基金引进人才项目(No.2019RC272) （No.2019RC272）
国家现代农业产业技术体系专项(No.CARS-32-20). （No.CARS-32-20）
