热带作物学报2024，Vol.45Issue(3)：622-631,10.DOI:10.3969/j.issn.1000-2561.2024.03.020
CsSSK1基因调控暹罗炭疽菌胁迫应答和致病性的功能分析
Functional Analysis of CsSSK1 Gene in Response to Stress Response and Virulence in Colletotrichum siamense
摘要
Abstract
Colletotrichum siamense is the main pathogen of anthracnose in many crops in tropical and subtropical re-gions.SSK1 is a response regulator protein of the two-component system.Studies have shown that SSK1 in pathogenic fungi is related to the morphogenesis,stress response,drug resistance and pathogenicity of pathogens,but SSK1 homo-logues have distinct functions in different fungi.In order to understand the function of CsSSK1 in C.siamense,the CsSSK1 gene deletion mutant ΔCsSSK1 and the complement strain ΔCsSSK1(CsSSK1)were constructed and pheno-typically observed.The results showed that the deletion mutant ΔCsSSK1 showed slightly retarded mycelial growth,shorter conidia,lower sporulation and conidial germination rate.ΔCsSSK1 significantly increased the sensitivity of C.siamense to NaCl,sorbitol,sucrose,glucose and Congo red,and the sensitivity to fluconazole and tebuconazole,but decreased the sensitivity to fludioxonil.The pathogenicity of ΔCsSSK1 was significantly reduced.The results showed that CsSSK1 gene was involved in the regulation of C.siamense morphogenesis,response to salt stress,osmotic stress,Congo red stress and fungicide stress,affecting the pathogenicity of C.siamense.The results would lay a foundation for further understanding the function of CsSSK1 gene in C.siamense and analyzing the molecular mechanism of patho-genic fungi response to stress.关键词
暹罗炭疽菌/CsSSK1基因/功能分析/药剂敏感性/致病性Key words
Colletotrichum siamense/CsSSK1 gene/functional analysis/fungicide sensitivity/virulence分类
农业科技引用本文复制引用
鲁婧文,关小灵,李潇,张宇,缪卫国,林春花..CsSSK1基因调控暹罗炭疽菌胁迫应答和致病性的功能分析[J].热带作物学报,2024,45(3):622-631,10.基金项目
海南省自然科学基金项目(No.320RC477) （No.320RC477）
国家自然科学基金项目(No.32160613) （No.32160613）
现代农业产业技术体系建设专项资金项目(No.CARS-33-BC1). （No.CARS-33-BC1）
