四川大学学报（自然科学版）2024，Vol.61Issue(2)：168-177,10.DOI:10.19907/j.0490-6756.2024.026003
ASI基因家族结构及表达分析
Structural and expression analysis of the ASI gene family
摘要
Abstract
To study the structural features and functions of genes encoding α-amylase/subtilisin inhibitor(ASI),a total of 33 ASI gene family members from 23 plant species were identified in this study by using NCBI,Phytozome and other platforms.The evolutionary characteristics the physicochemical properties and conserved motifs of ASI proteins were analyzed.At the same time,RT-qPCR was combined to analyze the expression pattern of RASI under drought and salinity stress.The results showed that the ASI gene family is highly conserved,but the upstream and downstream regions of the encoded proteins are differently conserved.Meanwhile,the cis-acting element prediction results suggested that the ASI genes may be involved in the physiological processes such as the regulation of plant growth and development,and the response to biotic and abiotic stresses.RT-qPCR analysis revealed that the expression of RASI in roots,leaf sheaths and leaves of rice at the seedling stage changed differently compared with the unstressed condition.These results indicate that ASI genes may have diverse biological functions.关键词
α-淀粉酶/枯草杆菌蛋白酶抑制剂(ASI)/Kunitz型抑制剂/基因家族鉴定/基因序列分析/基因表达分析Key words
α-amylase/subtilisin inhibitor,ASI/Kunitz-domain inhibitors/Gene family identification/Gene sequences analysis/Gene expression analysis分类
农业科技引用本文复制引用
黄斌翰,何艳艳,唐婕,叶春,周嘉裕,廖海..ASI基因家族结构及表达分析[J].四川大学学报（自然科学版）,2024,61(2):168-177,10.基金项目
国家自然科学基金(32270410) （32270410）
成都市科技局项目(2022-YF05-01357-SN) （2022-YF05-01357-SN）
中央高校医工结合项目(2682021ZTPY017,2682022ZTPY053) （2682021ZTPY017,2682022ZTPY053）
