食管疾病2024，Vol.6Issue(1)：1-7,7.DOI:10.15926/j.cnki.issn2096-7381.2024.01.001
牙龈卟啉单胞菌FimA促进ESCC细胞MHC-I的降解与免疫抑制
Virulence factors FimA of Porphyromonas Gingivalis Inhibits the Immunity by Triggering MHC-I Degradation
摘要
Abstract
Objective To investigate the impact and regulatory mechanisms of Pg infection on MHC-I molecules in esophageal squamous cell carcinoma(ESCC)cells.Methods Western blot was employed to assess the expression levels of the major virulence factor fimbriae(FimA)and MHC-I after Pg infection of KYSE-150 cells.Immunoprecipitation(Co-immunoprecipitation,Co-IP)was used to explore the interaction between FimA and MHC-I.Confocal microscopy was utilized to monitor changes in fluorescence following LC3 dual-fluorescence lentivirus transduction in KYSE150 cells.Western blot was conducted to measure the expression of LC3B-Ⅱ after Pg infection in KYSE150 cells.Flow cytometry was employed to evaluate Ki67 expression in Peripheral Blood Mononuclear Cells(PBMCs)following co-culture with Pg-infected KYSE150 cells.Results Western blot revealed increased expression of FimA and decreased expression of MHC-I after Pg infection of KYSE150 cells.Co-IP indicated a direct interaction between FimA and MHC-I proteins.Confocal microscopy demonstrated enhanced fluorescence intensity after Pg infection of KYSE150 cells.Flow cytometry showed significant reduction of Gzmb and Mki67 expression in PBMCs co-cultured with Pg-infected KYSE150 cells(both P<0.001).Conclusion The Pg virulence factor FimA may downregulate MHC-I expression through the autophagy pathway and suppress T-cell immune responses,thereby promoting the formation of an inhibitory immune microenvironment in ESCC and facilitating tumor progression.关键词
食管鳞状细胞癌/牙龈卟啉单胞菌/自噬/毒力因子/肿瘤免疫微环境Key words
esophageal squamous cell carcinoma/Porphyromonasgingivalis/autophagy/virulence factor/tumor immune microenvironment分类
医药卫生引用本文复制引用
张旭东,张秀森,杨泽,陈乐乐,丁剑兰,原翔..牙龈卟啉单胞菌FimA促进ESCC细胞MHC-I的降解与免疫抑制[J].食管疾病,2024,6(1):1-7,7.基金项目
河南省优秀青年科学基金项目(222300420041)河南省中青年卫生健康科技创新人才培养项目(YYKC2020040)河南省中原英才计划(育才系列) （222300420041）
