水生态学杂志2024，Vol.45Issue(3)：103-111,9.DOI:10.15928/j.1674-3075.202212260511
两种沉水植物及其叶面附着细菌对钙磷添加的响应研究
Response of Two Submerged Plants and Their Leaf Epiphytic Bacterial Community to the Addition of Calcium and Phosphorus
摘要
Abstract
The microenvironment produced by photosynthesis in submerged plants is conducive to co-precipitation of calcite(CaCO3)and phosphate(PO43-).However,the extent of CaCO3_PO4 co-precipitation varied among different submerged species.In this study,Potamogeton crispus and Elo-dea canadensis were selected for research,and we investigated the response of submerged plants and their leaf epiphytic bacteria to additions of Ca2+and PO43-to the water.The experiments were carried out in buckets(38 cm,30 cm)from December 22,2020 to January 8,2021.Six Ca2+and PO43-treat-ments(0 mg/L PO43-,0 mg/L Ca;0.2 mg/L PO43-,0 mg/L Ca;2.0 mg/L PO43-,0 mg/L Ca;0 mg/L PO43-,100 mg/L Ca;0.2 mg/L PO43-,100 mg/L Ca;2.0 mg/L PO43-,100 mg/L Ca)were set for each plant species,and a control group with no plants.Each treatment was run in triplicate,and water from each treatment was sampled every three days,beginning on Day 2,and the plants were collect-ed at the end of the experiment.Solution pH,total phosphorus(TP),soluble reactive phosphorus(SRP),chlorophyll(Chl-a)and total alkaline phosphatase activity(TAPA)were measured and plants were analyzed for total phosphorus(dry weight),TP and calcium phosphate(Ca-PO4)and the leaf epiphytic bacteria community was characterized.Results show that(1)the addition of Ca2+decreased the concentration of TP and SRP in water,and the concentration of TAPA had a negative correlation with pH.The content of Chl-a in P.crispus culture treatments was negatively correlated with pH,while Chl-a was positively correlated with pH in E.canadensis culture treatment.After adding Ca2+concentration of 100 mg/L,TAPA in overlying water of P.crispus treatments had a negative correla-tion with SRP and TP,while the correlation between TP and SRP in overlying water of E.canaden-sis and Chl-a was positive.(2)Total phosphorus in dry weight,total phosphorus in ash and calcium-phosphate in ash of P.crispus and E.canadensis increased with the increase of phosphorus concentra-tion.In 100 mg/L Ca2+adding treatments,total phosphorus in ash and Ca-P contents of P.crispus in-creased,while those of E.canadensis decreased.Ca-P concentration of P.crispus was higher than that of E.canadensis.(3)The relative abundance of Proteobacteria and Cyanobacteria were both the richest in the microbial communities attached to the leaves of P.crispus and E.canadensis,account-ing for 71％-84％of the total abundance.In the treatment of 2.0 mg/L P+0 mg/L Ca,the abundance of Proteobacteria on both species reduced,while that of Cyanophyta increased.After adding Ca2+concentration of 100 mg/L,the abundance of Proteobacteria on the leaves of P.crispus increased with the increase of phosphorus concentration,but the abundance of Cyanophyta decreased.For E.canadensis,the abundance of Proteobacteria changed little,while the abundance of Cyanophyta increased with the increase of phosphorus concentration.In conclusion,the Ca-P contents,the mor-phological structure of the leaves and the composition of leaf epiphytic bacteria showed significant in-terspecific differences due to the difference in co-precipitation capacity of calcium and phosphate.关键词
沉水植物/叶面附着细菌群落/灰分磷/钙磷Key words
submerged plant/leaf epiphytic bacterial community/phosphorus in ash/calcium-phosphorus分类
生物科学引用本文复制引用
王和云,胡睿,周易,陈矿,程华强,常锋毅..两种沉水植物及其叶面附着细菌对钙磷添加的响应研究[J].水生态学杂志,2024,45(3):103-111,9.基金项目
国家自然科学基金项目(41901135) （41901135）
长江科学院开放基金项目(CKWV2019770/KY) （CKWV2019770/KY）
湖北省教育厅项目(Q20191303). （Q20191303）
