天津中医药2024，Vol.41Issue(4)：495-502,8.DOI:10.11656/j.issn.1672-1519.2024.04.17
加减补阳还五汤通过SIRT1途径激活自噬改善糖尿病肾病小鼠肾组织损伤
Modified Buyang Huanwu Decoction improves renal tissue damage in diabetic kidney disease mice by activating autophagy through SIRT1 pathway
摘要
Abstract
[Objective]To investigate whether the modified Buyang Huanwu Decoction can improve the renal tissue damage of diabetic kidney disease(DKD)mice by activating autophagy through SIRT1 pathway.[Methods]The mice with DKD were randomly divided into model group and traditional Chinese medicine group,and the db/m group was regarded as the normal group.After 8 weeks of administration,the levels of fasting blood glucose(FBG),glycosylated hemoglobin(HBA1c),total cholesterol(TC),triglyceride(TG),urea nitrogen(BUN),creatinine(Scr)and urinary microalbumin(mALB)were measured.The expression of wilms tumor 1 protein(WT1),nephrin and podocin in mouse kidney tissue were detected by qRT-PCR and immunohistochemistry.Western blot was used to detect the protein expressions of silent information regulator 1(SIRT)1,human microtubule-associated protein light chain 3B(LC3B),autophagy-related protein 7(Agt7),Sequestosome 1(P62),Beclin1,B-cell lymphoma-2(Bcl-2),BCL-2-associated X protein(Bax)and Caspase-3 in mice kidney tissue.At the same time,the pathological changes of kidney were observed under light microscope.[Results]After Chinese medicine intervention,the renal pathological changes in mice were lighter than those in the model group.The levels of mALB,blood lipids(TC,TG)were decreased.The renal function(BUN,Scr)was improved.At the same time,it inhibited the expressions of P62,Bax and Caspase-3 in renal tissue,up-regulated the expression of SIRT1,Beclin-1,Agt7,LC3B-Ⅱ/LC3B-I and Bcl-2.It also improved the expression of podocyte marker proteins WT1,nephrin and podocin in renal tissue(P＜0.05 or P＜0.01),but had no significant effect on blood glucose levels(P＞0.05).[Conclusion]The modified Buyang Huanwu Decoction can exert a renal protective effect by enhancing autophagy,inhibiting apoptosis and protecting podocytes in mouse kidney tissue.Among them,the SIRT1 pathway may be its potential way to promote autophagy.关键词
糖尿病肾病/加减补阳还五汤/沉默信息调节因子1/自噬/凋亡Key words
diabetic kidney disease/modified Buyang Huanwu Decoction/silent information regulator 1/autophagy/apoptosis分类
医药卫生引用本文复制引用
杨帆,张晓云,高俊侠,刘利飞,黄家安,王开爽,王月华..加减补阳还五汤通过SIRT1途径激活自噬改善糖尿病肾病小鼠肾组织损伤[J].天津中医药,2024,41(4):495-502,8.基金项目
河北省中医药管理局科研计划项目(2023119,2022108) （2023119,2022108）
河北省自然科学基金项目(H2022423320,H2022423342) （H2022423320,H2022423342）
大学生创新创业计划项目(202314432020). （202314432020）
