天津中医药2024，Vol.41Issue(4)：510-516,7.DOI:10.11656/j.issn.1672-1519.2024.04.19
藏红花素抑制铁死亡改善糖尿病肾病的机制研究
Mechanism of crocin treatment on improving diabetic nephropathy by inhibiting ferroptosis
摘要
Abstract
[Objective]To investigate the effect of crocin(CRO)on diabetic nephropathy(DN)and explore its mechanism from the perspective of lipid peroxidation and ferroptosis.[Methods]Sixty C57BL/6J mice were adaptively fed for 1 week and then HFD for 8 weeks.After STZ modeling,the mice were randomly divided into control group,model group,ferroptosis inhibitor group(Fe-1,10 mg/kg,gavage),low dose CRO group(CROL,5 mg/kg,gavage),middle dose CRO group(CROM,10 mg/kg,gavage),high dose CRO group(CROH,20 mg/kg,gavage).After 8 weeks of treatment,24 h urine samples,blood samples and kidneys were collected for analysis and determination.The levels of 24 h-UPQ,Cr and BUN in serum,MDA,ROS and 4-HNE in renal tissue were measured by kits to evaluate renal function and lipid peroxidation.HE,Masson and TUNEL staining were used to observe the pathological changes and cell death in the same part of the kidney.Level of Fe in renal was measured,and the expressions of STEAP3,TF,FTH1,FTL,and GPX4 were detected by RT-qPCR and Western blot to evaluate the effect of CRO on ferroptosis.[Results]Compared with the model group,the levels of Cr,BUN,24 h-UPQ decreased different degrees,and the pathological damage of renal tissue has different degrees of improvement,and the levels of MDA,ROS,4-HNE were significantly reduced,and TUNEL fluorescence positive reaction in renal tissue were significantly weakened,and the level of Fe in renal tissue decreased in different degrees after CRO treatment.The expression of STEAP3 and TF was down-regulated,while the expression of FTH1,FTL and GPX4 was up-regulated.[Conclusion]CRO can protect the kidney of DN mice by inhibiting lipid peroxidation and reducing ferroptosis.关键词
藏红花素/糖尿病肾病/脂质过氧化/铁死亡Key words
crocin/diabetic nephropathy/lipid peroxidation/ferroptosis分类
医药卫生引用本文复制引用
陈晓婷,潘保朝,王元松,苏金浩,陈伟,及晓晖,吕树泉..藏红花素抑制铁死亡改善糖尿病肾病的机制研究[J].天津中医药,2024,41(4):510-516,7.基金项目
河北省中医药管理局课题(2024458). （2024458）
