基于UPLC指纹图谱与多成分定量评价不同产地桑叶药材质量OACSTPCD

[Objective]To establish ultra-high-performance liquid chromatography(UPLC)fingerprint of Mori Folium and to comprehensively evaluate the quality of Mori Folium from different origins by combining chemo-metrics and multi-component content determination.[Methods]A CORTECS UPLC C18 column(2.1 mm×100 mm,1.6 μm)chromatographic column was employed in this study,gradient elution was performed using 0.1%formic acid water(A)-acetonitrile(B)as the mobile phase,and the detection wavelength was 350 nm.UPLC fingerprints of 16 batches of Mori Folium were established,and the contents of components were determined.The fingerprints were evaluated by similarity and principal component analysis,and partial least squares-discriminant analysis was performed to find the differential components of Mori Folium from different origins.[Results]A total of 14 common peaks were calibrated in the fingerprint of Mori Folium,and 8 of them were identified by comparison with the standards,namely,neochlorogenic acid,chlorogenic acid,caffeic acid,cryptochlorogenic acid,rutin,isoquercitrin,nicotiflorin and astragalin.The similarity of 16 batches was greater than 0.962.Principal component analysis classified the 16 batches of Mori Folium into three categories,and partial least squares-discriminant analysis screened out five markers,with the significance of differences ranked as peak 1(neochlorogenic acid)>peak 4(cryptochlorogenic acid)>peak 3(caffeic acid)>peak 14>peak 11.[Conclusion]The established comprehensive evaluation method is stable and reliable,which can provide a theoretical basis for the quality evaluation study of Mori Folium.