一种灰茶尺蠖高效引诱剂的研制及其田间防效评价OA北大核心CHSSCDCSTPCD

In order to develop a sex pheromone/plant-derived volatile complex sexual attractant for efficaciously trapping Ectropis grisescens female and male adults,thirty major volatile compounds from tea shoots and tea flowers were chosen and prepared as odor sources at the dosages of 10-6 g/mL,10-4 g/mL,and 10-2 g/mL in liquid paraffin.Each dosage treatment was applied for electroantennogram(EAG)determination on 1 day-old male and female adults of E.grisescens.EAG responses showed that:① Every dosage could elicit obvious EAG responses,and EAG value significantly increased with the rise of the dosage;② The female and male adults showed similiar EAG response values.③ Each dosage treatment of linalool,trans-2-hexenal,cis-3-hexen-1-ol,hexanol,phenethyl alcohol,and 6-methyl-5-hepten-2-one caused intense EAG responses.The six EAG-active components blended with two main sex pheromones,Z,Z,Z-3,6,9-octadecatriene,and Z,Z-3,9-6,7-epoxy-octadecadiene,to make up the various candidates of the complex sexual attractant.Every candidate was uploaded onto a little strip of wool felt,which was sealed by PVC film to compose a trap for field trials.After a series of trials,a candidate,i.e.complex seaxual attractant,gave the optimal control effect.Its composition on one lure was that 2 g of each of the six EAG-active compounds was blended with 0.4 mg Z,Z,Z-3,6,9-octadecatriene,0.6 mg Z,Z-3,9-6,7-epoxy-octadecadiene,1 mg butylated hydroxy toluene,and 100 μL hexane.In addition,the attracting effect of thirteen colored types of sticky boards,including earth yellow,fluorescent yellow,beige yellow,jasmine yellow,orange,clay brown,dark green,bud green,grass green,fluorescent green,sky blue,bright red and pure white,on the geometrid adults was tested in tea plantations,and the adults preferred dark yellow to other colors with the earth yellow appeared optimum.One lure attached on one earth yellow sticky board to form one complex sexual attractant trap.The most serious damaging generation of the geometrid was the 3rd generation.During the 2nd generation eclosion peak beginning stage,the traps were applied for mass trapping its adults,so as to reduce mating opportunity and lower the individual number of the third generation larvae.Both 2.5％bifenthrin,and Ectropis obliqua nucleopolyhedrovirus(Ecob NPV)·Bacillus thuringiensis preparations(containing both 1×104 PIB/μL Ecob NPV and 2000 IU/μL Bacillus thuringiensis)were respectively spayed on the larvae of the 3rd generation.In turn,the control effect of each of three treatments was investigated at an interval,and that of complex sexual attractant exceled the latter two.The present study provide the applicable technology and product for green control on the geometrid.