生态学杂志2024，Vol.43Issue(3)：644-655,12.DOI:10.13292/j.1000-4890.202403.034
丛枝菌根真菌接种与磷添加对干旱胁迫燕麦土壤微生物生物量及酶活性的影响
Effects of AMF and phosphorus application on microbial biomass and enzyme activities in oat soil under drought stress
摘要
Abstract
The objectives were to investigate the effects of applying AMF and phosphorus on AMF infection of oat roots,soil microbial biomass,soil enzyme activity,as well as oat yield under drought stress,so as to develop AMF and phosphorus application guidelines for growing oat under drought.The experiment was conducted in pots for a total of 12 treatments,consisted of two levels of water supply,normal water supply(75％soil relative water con-tent,W1)and drought stress(55％soil relative water content,W2),three levels of phosphorus supplement,0,20,and 40 mg·kg-1(P0,P1,P2),and two levels of AMF,inoculated(AMF)and not inoculated(NAMF).Root and soil samples were collected at the jointing,filling,and maturity stages to determine the AMF infection rate of oat roots,the soil MBC,MBN,and MBP,the soil sucrase,urease,and alkaline phosphatase activities.Oat yield was determined at maturity stage.The results showed that significant effects of soil water content,phosphorus,and AMF treatment were found on these indicators.Significant interactions among three factors were found on soil MBN and soil sucrose activities.Under drought stress,compared with non-inoculated AMF,inoculated AMF signifi-cantly increased these indicators.Compared to P0,the AMF infection rate,the soil MBC,MBN,and MBP,the soil sucrase,urease,and alkaline phosphatase activities and yield significantly increased by 13.21％,52.26％,47.07％,88.94％,23.15％,15.44％,11.15％and 17.16％(the maximum value)with P1 application in oat growth period,respectively.However,these indicators tended to be reduced with further increasing of phosphorus application(P2).Therefore,AMF inoculation and a moderate application of phosphorus were effective ways to increase the soil MBC,MBN and MBP,improve the activities of soil sucrase,urease and alkaline phosphatase,and increase oat yield under drought stress;however,the oat yield did not reach the level in normal water supply without AMF inoculation.关键词
燕麦/干旱胁迫/AMF/磷/土壤微生物生物量/土壤酶活性Key words
oat/drought stress/AMF/phosphorus/soil microbial biomass/soil enzyme activity引用本文复制引用
张斌,吕玉峰,李利,冯美臣,王超,宋晓彦,杨武德,张美俊..丛枝菌根真菌接种与磷添加对干旱胁迫燕麦土壤微生物生物量及酶活性的影响[J].生态学杂志,2024,43(3):644-655,12.基金项目
山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目(20220401091002-1-2)、山西省重点研发计划项目(201903D221061)和2021年山西省研究生教育创新项目(2021Y334)资助. （20220401091002-1-2）
