生物加工过程2024，Vol.22Issue(2)：189-195,7.DOI:10.3969/j.issn.1672-3678.2024.02.009
HPLC法测定五味子颗粒中7种木脂素成分的含量
Measurement of seven lignans in Schisandrae Chinensis Fructus Granules by HPLC
摘要
Abstract
A reliable HPLC method was established to measure the contents of 7 lignans in Schisandrae Chinensis Fructus Granules.The chromatographic process was performed on a Poroshell 120 SB-C18 column(150 mm×4.6 mm,2.7 μm)using gradient elution with the mobile phase of water-containing tetrahydrofuran and acetonitrile.With the optimized separation conditions(flow rate:0.6 mL/min;column temperature:30℃;detection wavelength:220 nm;injection volume:10 μL),the LODs for schizandrin,Gomisin A,Schisantherin A,Schisantherin B,Schisandrin A,Schisandrin B and Schisandrin C were found to be 0.017,0.041,0.156,0.165,0.136,0.166 and 0.113 μg/mL,respectively.Excellent linearity(r＞0.999 9)was achieved for 7 studied lignans,and the average extraction recoveries(RSD＜3.0％)ranged from 98.1％to 100.9％.Overall,our HPLC method demonstrated good reproducibility and high sensitivity,and provided an excellent tool in controlling the quality of Schisandrae Chinensis Fructus Granules,especially in identifying the harmful addition of Schisandrae Sphenantherae Fructus.In addition,this quantitative analysis approach laid a foundation for the revision of quality standards with regards to other products containing Schisandrae Sphenantherae Fructus.关键词
高效液相色谱/五味子颗粒/木脂素/定量分析Key words
high performance liquid chromatography(HPLC)/Schisandrae Chinensis Fructus Granules/lignan/quantitative analysis分类
医药卫生引用本文复制引用
陈亚运,雷棋,屈蓉..HPLC法测定五味子颗粒中7种木脂素成分的含量[J].生物加工过程,2024,22(2):189-195,7.基金项目
2021年江苏省大学生创新创业训练计划(202112106025Y) （202112106025Y）
"泰州市凤城英才"青年科技人才托举工程(202116) （202116）
