HPLC法测定五味子颗粒中7种木脂素成分的含量OACSTPCD

A reliable HPLC method was established to measure the contents of 7 lignans in Schisandrae Chinensis Fructus Granules.The chromatographic process was performed on a Poroshell 120 SB-C18 column(150 mm×4.6 mm,2.7 μm)using gradient elution with the mobile phase of water-containing tetrahydrofuran and acetonitrile.With the optimized separation conditions(flow rate:0.6 mL/min;column temperature:30℃;detection wavelength:220 nm;injection volume:10 μL),the LODs for schizandrin,Gomisin A,Schisantherin A,Schisantherin B,Schisandrin A,Schisandrin B and Schisandrin C were found to be 0.017,0.041,0.156,0.165,0.136,0.166 and 0.113 μg/mL,respectively.Excellent linearity(r＞0.999 9)was achieved for 7 studied lignans,and the average extraction recoveries(RSD＜3.0％)ranged from 98.1％to 100.9％.Overall,our HPLC method demonstrated good reproducibility and high sensitivity,and provided an excellent tool in controlling the quality of Schisandrae Chinensis Fructus Granules,especially in identifying the harmful addition of Schisandrae Sphenantherae Fructus.In addition,this quantitative analysis approach laid a foundation for the revision of quality standards with regards to other products containing Schisandrae Sphenantherae Fructus.