兽类学报2024，Vol.44Issue(2)：159-170,12.DOI:10.16829/j.slxb.150807
藏羚交配群成员间亲缘关系及遗传多样性分析
Parentage testing and genetic diversity of Tibetan antelope individuals in or among mating groups
摘要
Abstract
Tibetan antelope(Pantholops hodgsonii),an endemic species of the Qinghai-Tibet Plateau,exhibits group liv-ing and sexual segregation behaviors.Outside the rutting season,male and female Tibetan antelope live separately.In this study,a total of 188 fresh fecal samples were collected from 32 groups of Tibetan antelope in the Kekexili region of the Sanjiangyuan National Park in late December 2021.Genetic diversity analysis and parentage testing were conducted using ten highly polymorphic microsatellite loci.The findings revealed that out of the 188 fresh fecal samples,genetic identification confirmed the presence of 145 individual Tibetan antelope.Among them,ten Tibetan antelope individuals(eight females and two males)sequentially appeared in different groups over time.Field observations indicated three mechanisms for recombination among mating groups including group dissolution,male departures or arrivals to/from ex-isting groups,and female departures or arrivals to/from existing groups.Notably,there was considerable genetic differen-tiation between individuals that changed groups compared to those remaining within their original group composition.Male Tibetan antelopes moved to groups with a higher proportion of females than their previous ones,thereby increasing their potential for acquiring more mates and enhancing opportunities for gene inheritance.The average number of alleles across the ten microsatellite loci was 16.1,with an average polymorphic information content of 0.766.Observed hetero-zygosity(Ho)ranged from 0.607 to 0.993 with a mean value of 0.819.Expected heterozygosity(He)ranged from 0.575 to 0.930 with a mean value of 0.798.These results indicated a high level of genetic diversity within the Tibetan antelope population.At the group level,out of the total parent-child relationships observed(n=32),fourteen(43.75％)occurred within the same group,predominantly in mother-daughter relationships(71.43％),while only four(28.57％)involved males(mother-son/father-daughter/father-son).By comparing the average coefficients of relatedness between groups with or without males,our findings suggested that the presence or absence of males had minimal impact on genet-ic relatedness among group members,indicating that closest genetic relationships within mating groups were primarily among females.Our findings demonstrated that low levels of inbreeding in Tibetan antelope populations and highlight female-female associations as key drivers for forming stable groups and facilitating communication and transmission of migration information due to their higher relatedness compared to males.These results provided scientific evidence sup-porting further validation of the collective memory conjecture regarding Tibetan antelope migration.关键词
藏羚/粪便DNA/交配群/亲缘关系/遗传多样性Key words
Tibetan antelope/Fecal DNA/Mating group/Parentage testing/Genetic diversity分类
生物科学引用本文复制引用
赛青高娃,王子涵,李全邦,王东,连新明..藏羚交配群成员间亲缘关系及遗传多样性分析[J].兽类学报,2024,44(2):159-170,12.基金项目
国家重点研发计划项目(2023YFF1305000) （2023YFF1305000）
青海省自然科学与工程技术学科带头人专项资金 （）
中国科学院"西部之光"青年学者A类项目 （）
