畜牧与兽医2024，Vol.56Issue(4)：108-116,9.
绵羊源荚膜血清D型多杀性巴氏杆菌的生物学特性及2种候选疫苗的免疫效果评价
Biological characterization of Pasteurella multocida capsular serotype D from sheep and immune evaluation of two candidate vaccines
摘要
Abstract
This study was to identify the biological characteristics of Pasteurella multocida capsular serotype D(PmD)from sheep in the Xinjiang area,and to explor the immune protection effect of its inactivated vaccine and the OmpH recombinant vaccine in mice,in order to provide reference measures for the prevention and control of sheep pasteuriasis in Xinjiang.The biological characteristics of PmD were pre-liminarily clarified by bacterial isolation and identification,PCR identification,drug susceptibility test,drug resistance and virulence gene detection,and by pathogenicity test.PmD inactivated vaccine and PmD-OmpH recombinant vaccine were prepared using methods such as optimal inactivation conditions,adjuvant screening,safety test and prokaryotic expression.Then,mouse challenge protection test was used to evaluate the immune efficacy of both the vaccines.One strain of PmD was isolated from sheep lung,and drug susceptibility tests showed that the bacterium resisted against 7 resistant drugs at the same time,but no resistance gene was detected.The virulence gene test showed that the bacteriium had 12 virulence genes(exbB,exbD,hgbA,tonB,fimA,Oma87,OmpH,Psl,sodA,sodC,tbpA,and toxA).Its pathogenicity was strong,and the minimum lethal quantity in mice was 2.2×105 CFU.The protection rates of the inactivated vaccine and recombinant vac-cine against the same serotype strain were 80%and 10%,respectively.The protection rates against different serotypes(A,F,unknown)strains were 0,20%,0,0,0,and 0.In this study,a strong drug-resistant and virulent sheep-derived PmD was identified.The inactivated vaccine was much more protective against the same serotype strain than the recombinant vaccine.In sheep farms in the disease area,the inac-tivated vaccine could be used to prevent and control pasteulosis in sheep.The development of the multivalent vaccines and new vaccines still requires further exploring.关键词
绵羊/荚膜血清D型/多杀性巴氏杆菌/生物学特性/灭活疫苗/OmpH蛋白/重组疫苗Key words
sheep/capsular serotype D/Pasteurella multocida/biological characteristics/inactivated vaccines/OmpH protein/recombi-nant vaccine分类
农业科技引用本文复制引用
汪阳,韩翔舒,夏俊,张凌,金映红,汪萍,薛晶,梁芊芊,李晓卓,郑启铭,刘文锴..绵羊源荚膜血清D型多杀性巴氏杆菌的生物学特性及2种候选疫苗的免疫效果评价[J].畜牧与兽医,2024,56(4):108-116,9.基金项目
新疆维吾尔自治区中央引导地方项目(ZYYD2022B16) （ZYYD2022B16）
新疆维吾尔自治区重大科技专项项目(2022273334) （2022273334）
新疆维吾尔自治区天山英才青年科技拔尖人才项目(2022196128) （2022196128）
