畜牧与兽医2024，Vol.56Issue(4)：117-124,8.
金樱子多糖、车前子多糖和冬葵果多糖体内外抗炎效果比较
Comparison of the in vivo and in vitro anti-inflammatory effects of Rosa laevigata Michx polysaccharide,Plantaginis Semen Growth polysaccharide and Fructus Malvae polysaccharide
摘要
Abstract
This study aimed to compare anti-inflammatory activities of Rosa laevigata Michx polysaccharide,Plantaginis Semen Growth pol-ysaccharide,and Fructus Malvae polysaccharide in vivo and in vitro.Firstly,a RAW264.7 cell model was established using lipopolysaccha-ride(LPS)for estimation of inflammation indicators in vitro,which were treated with Rosa laevigata Michx polysaccharide,Plantaginis Semen Growth polysaccharide,and Fructus Malvae polysaccharide,respectively.Subsequently,RAW264.7 cell viability was detected by MTT assay.The level of nitric oxide(NO)was tested by Griess assay.The content of interleukin 6(IL-6),interleukin 1β(IL-1β),and α-tumor necrosis factor(TNF-α)in supernatants were tested by enzyme-linked immunosorbent assay(ELISA).The mRNA expression of TNF-α,IL-6,and IL-1β was tested by RT-PCR assay.Secondly,a DSS-induced ulcerative colitis mouse model was used in experiments in vivo.Polysaccharides(200 mg/kg),5-aminosalicylic acid(200 mg/kg),and saline were given to the polysaccharides group,the posi-tive drug groups,the normal group,and the model group(DSS),respectively,by gavage.TNF-α,IL-6,and IL-1β levels in colonic tis-sues were determined using ELISA.Histopathologic change in the colon was analyzed by measuring colon length and calculating histopatholog-ic scores.In vitro,the Rosa laevigata Michx polysaccharide group had the most effective inhibitory effect on the content of NO,TNF-α,IL-6,and IL-1β and the mRNA expression of pro-inflammatory factors(TNF-α,IL-6,IL-1β),followed by the Plantaginis Semen Growth polysaccharide group,whereas the Fructus Malvae polysaccharide group had no significant effect in inhibiting the growth of inflamma-tory mediators,compared with the model group(LPS)at the same concentrations.In vivo,compared with the model group(DSS),the dis-ease activity index(DAI)score(P＜0.05),histopathological score(P＜0.001),and levels of TNF-α,IL-6,and IL-1β(P＜0.001)in the colon tissues were alleviated,and the colon length and the level of IL-10(P＜0.01)were modified in the Rosa laevigata Michx polysac-charide group.Notably,the Plantaginis Semen Growth polysaccharide group had a similar result which included the histopathological score(P＜0.05),the levels of TNF-α,IL-6,and IL-1β(P＜0.05)in colon tissues,which could be alleviated.The colon length(P＜0.05)and the level of IL-10(P＜0.01)were modified.However,the Fructus Malvae polysaccharide group showed no effect in relieving colitis.In summary,compared with Plantaginis Semen Growth polysaccharide and Fructus Malvae polysaccharide,Rosa laevigata Michx polysaccharide had the best anti-inflammatory effects and could be a candidate drug of polysaccharide anti-inflammatory agents.关键词
金樱子多糖/冬葵果多糖/车前子多糖/炎症因子/抗炎Key words
Rosa laevigata Michx polysaccharide/Plantaginis Semen Growth polysaccharide/Fructus Malvae polysaccharide/inflammatory factors/anti-inflammation分类
农业科技引用本文复制引用
彭松,卢炫圻,林芳竹,毛宁宁,于亚明,于琳,施宗傲,王德云..金樱子多糖、车前子多糖和冬葵果多糖体内外抗炎效果比较[J].畜牧与兽医,2024,56(4):117-124,8.基金项目
国家重点研发计划项目(2022YFD1801103) （2022YFD1801103）
中央高校基本科研业务费专项(KYCXJC2023003) （KYCXJC2023003）
