刑事技术2024，Vol.49Issue(2)：135-140,6.DOI:10.16467/j.1008-3650.2023.0081
气相色谱-三重四极杆质谱测定全血中5种常见兽用麻醉剂
Determination of Five Common Veterinary Anesthetics in Whole Blood by Gas Chromatography-triple Quadrupole Mass Spectrometry
摘要
Abstract
An analytical method was established for determination of 5 common veterinary anesthetics(tiletamine,xylazole,ketamine,xylazine and zolazepam)in whole blood by using programmed temperature vaporizer(PTV)system combined GC-MS/MS.Those samples were dealt with salting-out-assisted liquid-liquid extraction(SALLE),introducing NaCl as salting-out regent and acetonitrile as extraction solvent.After that,the extracting solution was purified by QuEChERS nano column.Most acetonitrile in the solution were removed by PTV system before analyzing in GC-MS/MS for protection of gas chromatographic column.The multiple-reaction-monitoring(MRM)positive ion mode was selected for detection.Three pairs of characteristic ions were taken for qualitative analysis and external standard method was accepted for quantitative analysis.The method was verified well.The results showed that the LODs were 10-20 ng/mL and LOQs were 20-40 ng/mL.There were good linear relationships for all the analytes with correlation coefficients(r)not less than 0.998.At three spiked levels of 40,400,and 2 000 ng/mL,the recoveries for 5 veterinary anesthetics in whole blood ranged from 71.61％to 119.10％.The intra-day precision and inter-day precision of the method evaluated at the concentration of three levels ranged from 0.34％to 14.81％,4.03％to 14.87％,respectively.The developed method with broad research potential benefited its simple pretreatment,fine stability and highly sensitivity,which was applied in an actual case.关键词
兽用麻醉剂/气相色谱-三重四极杆质谱/程序升温进样/盐析辅助液液萃取/QuEChERS技术Key words
veterinary anesthetics/GC-MS/MS/PTV/SALLE/QuEChERS分类
社会科学引用本文复制引用
董林沛,任昕昕,李佳宜,董梅..气相色谱-三重四极杆质谱测定全血中5种常见兽用麻醉剂[J].刑事技术,2024,49(2):135-140,6.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(2022JB003) （2022JB003）
