中国免疫学杂志2024，Vol.40Issue(3)：449-455,7.DOI:10.3969/j.issn.1000-484X.2024.03.001
弹性蛋白酶调节中性粒细胞炎性募集的功能研究
Functional study on elastase regulation of inflammatory neutrophils recruitment
摘要
Abstract
Objective:To explore the effect of neutrophil elastase(NE)on neutrophil inflammatory recruitment.Methods:Mice bone marrow-derived neutrophils were pretreated with an exogenous elastase inhibitor-sivelestat sodium.The effects of elastase inhibition on the in vivo inflammatory recruitment,chemotaxis,adhesion,cell polarization and spreading of NE were examined by peritonitis adoptive transfer assay,dunn chamber,flow chamber,immunofluorescence staining and spreading assay,respectively.The effects of elastase inhibition on NE phagocytosis and reactive oxygen species(ROS)release capacity were detected by flow cytom-etry and the luminol chemiluminescence system.Results:Sivelestat sodium pretreatment significantly attenuated neutrophil in vivo in-flammatory recruitment(P<0.001);impaired neutrophil perception of chemotaxis in vitro(P<0.05),slowed chemotactic velocity(P<0.05),and decreased the chemotactic distance(P<0.05);reduced the adhesion of neutrophils to inflamed endothelial cells(P<0.000 1)and inhibited the phagocytosis of bacteria by neutrophils(P<0.01);however,there was no significant effect on neutrophil spreading,polarization and ROS.Conclusion:NE inhibition significantly impaired the inflammatory recruitment cascade response and phagocyto-sis of neutrophils in vitro and in vivo but had no significant effect on the spreading,polarization and ROS release of neutrophils.关键词
中性粒细胞/弹性蛋白酶/西维来司钠盐/炎性募集Key words
Neutrophils/Elastase/Sivelestat/Inflammatory recruitment分类
医药卫生引用本文复制引用
刘洋,孟玲,范思佳,任春光,张欢..弹性蛋白酶调节中性粒细胞炎性募集的功能研究[J].中国免疫学杂志,2024,40(3):449-455,7.基金项目
中国博士后科学基金面上项目(2021MD703921) （2021MD703921）
重庆市人社局重庆市留学人员回国创业创新支持计划(cx2020029) （cx2020029）
重庆市科委博士直通车项目CSTB2022BSXM-JCX0036) （）
重庆市博士后科学基金(cstc2021jcyj-bshX0080) （cstc2021jcyj-bshX0080）
重庆医科大学未来医学青年创新团队发展支持计划(W0052). （W0052）
