中国免疫学杂志2024，Vol.40Issue(3)：456-460,465,6.DOI:10.3969/j.issn.1000-484X.2024.03.002
基于IL-6/JAK2/STAT3信号通路探讨灰树花提取物对溃疡性结肠炎大鼠结肠组织炎症反应的影响
Exploring effect of Grifola frondosa extract on inflammatory response of colonic tissue in rats with ulcerative colitis based on IL-6/JAK2/STAT3 signaling pathway
摘要
Abstract
Objective:To investigate the effect and mechanism of Grifola frondosa extract on inflammatory response of colon tissue in rats with ulcerative colitis(UC)by regulating interleukin-6(IL-6)/Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods:Forty SD rats were randomly divided into blank control group,UC model group,Grifola frondosa treatment group,western medicine treatment group and combined treatment group,with 8 rats in each group.After UC rats were established by free drinking 3%DSS for 7 days,the treatment group were given Grifola frondosa extract 10 mg/(kg·d),sulfasalazine 0.3 g/(kg·d),and the same amount of two drugs,for 14 consecutive days.During the experiment,general state of rats were observed,and the disease activity index(DAI)score was calculated;pathological changes of rats colon tissue were observed by HE staining;protein expression levels of IL-6,JAK2,STAT3 and p-STAT3 in rats colon tissue were detected by Western blot;content of IL-6 in rats serum was detected by ELISA;protein contents and expressions of IL-6R and MPO in rats colon tissue were determined by immunohistochemistry.Results:Compared with blank control group,general state of rats in UC model group was poor,DAI score was increased,obvious tissue mucosal defects and inflammatory cell infiltration were observed by HE staining;protein expression levels of IL-6,JAK2,STAT3 and p-STAT3 in rats colon tissue and contents of IL-6R and MPO were significantly increased(P<0.01);content of IL-6 in rats serum was significantly increased(P<0.01),the difference was statistically significant.Compared with UC model group,general condition of rats in each treatment group was improved,DAI score was decreased,HE staining showed that mucosal defects were improved to varying degrees,and occasionally inflammatory cell infiltration was observed;protein expression levels of IL-6,JAK2,STAT3 and p-STAT3 in colon tissue were significantly decreased(P<0.01),contents of IL-6R and MPO in colon tissue and content of IL-6 in serum were significantly decreased(P<0.01 or P<0.05),the differences were statistically significant.Conclusion:Grifola frondosa extract can reduce the inflammatory response in colon tissue of UC rats by regulating expressions of IL-6/JAK2/STAT3 signaling pathway related factors.关键词
灰树花提取物/溃疡性结肠炎/白细胞介素6/蛋白酪氨酸激酶2/信号转导及转录激活蛋白3Key words
Grifola frondosa extract/Ulcerative colitis/IL-6/Janus kinase 2/Signal transducer and activator of transcription 3分类
医药卫生引用本文复制引用
金雪,马贤德,赵卓,徐铭,王建光,杜晗,关洪全,韩晓伟..基于IL-6/JAK2/STAT3信号通路探讨灰树花提取物对溃疡性结肠炎大鼠结肠组织炎症反应的影响[J].中国免疫学杂志,2024,40(3):456-460,465,6.基金项目
国家自然科学基金项目(81403208). （81403208）
