中国免疫学杂志2024，Vol.40Issue(3)：461-465,5.DOI:10.3969/j.issn.1000-484X.2024.03.003
白细胞介素-12促进氧化应激参与干燥综合征肝脏病变
Interleukin-12 participate in liver diseases of Sjögren's syndrome through promoting oxidative stress
摘要
Abstract
Objective:To investigate the effect of interleukin-12(IL-12)on liver lesions and oxidative stress pathway in Sjo-gren's syndrome mice,and to clarify the possible mechanism of liver lesions in Sjogren's syndrome.Methods:Saliva flow rate,liver function,liver pathology and IL-12 level were measured in NOD,IL-12 knockout(IL-12KO)NOD and C57BL/6(B6)mice.Different concentrations of IL-12 and JAK2,TYK2 inhibitors were applied to mouse hepatoma cells.The oxidative stress index of mouse serum and cell culture supernatants of each group were determined.Results:Compared with the other two groups of mice,the NOD mice had significantly higher GOT and GPT(P<0.05),and decreased serum GSH-PX,SOD and CAT(P<0.05).CAT,GSH,GSH-PX and SOD were decreased,while MDA was increased in mice treated with different concentrations of IL-12.JAK2/TYK2 inhibitors reversed regulatory effects of IL-12 on SOD,GSH and MDA in hepatoma cells(P<0.05).Conclusion:IL-12 may aggravated liver damage of Sjögren's syndrome through promoting oxidative stress of hepatocytes.关键词
干燥综合征/肝脏病变/白细胞介素-12/氧化应激Key words
Sjögren's syndrome/Liver disease/Interleukin-12/Oxidative stress分类
医药卫生引用本文复制引用
查洁,江婷婷,郭俊巧,肖帧,姚根宏..白细胞介素-12促进氧化应激参与干燥综合征肝脏病变[J].中国免疫学杂志,2024,40(3):461-465,5.基金项目
国家自然科学基金面上项目(81970062) （81970062）
南京市医学科技发展资金项目(ZKX21027) （ZKX21027）
安徽省高校自然科学研究项目(2023AH053426). （2023AH053426）
