中国免疫学杂志2024，Vol.40Issue(3)：471-477,7.DOI:10.3969/j.issn.1000-484X.2024.03.005
糖尿病肾病小鼠CD4+T细胞的脂质组学研究
Study on lipidomics of CD4+T cells in mice with diabetic kidney disease
摘要
Abstract
Objective:To investigate the lipidomics differences of CD4+T immune cells in diabetic kidney disease(DKD)mice,and screen out the differential metabolites with biological significance.Methods:CD4(L3T4)MicroBeads immunomagnetic beads were used to isolate CD4+T immune cells from spleen of BKS.Cg-Dock7m+/+Leprdb/J mice with spontaneous DKD;the purity of CD4+T cells were identified by flow cytometry.The non-targeted lipidomics of CD4+T cells were detected by LC-MS/MS,and the differ-ential metabolites were analyzed.Results:A total of 463 metabolites were detected by LC-MS.PCA and OPLS-DA analysis showed that the metabolic components were significantly separated;twenty-four differential metabolites were screened out.KEGG and enrich-ment analysis showed that the differential metabolites involved in the disorder of glycerol phospholipid metabolism.Conclusion:Phos-pholipid metabolism of CD4+T cells is closely related to the occurrence of DKD.Phospholipid metabolism targeting DKD CD4+T cells in DKD may be a new direction of DKD treatment.关键词
CD4+T细胞/糖尿病肾病/脂质组学/磁珠分选/流式细胞术Key words
CD4+T cells/Diabetic kidney disease/Lipidomics/Magnetic bead sorting/Flow cytometry分类
医药卫生引用本文复制引用
张小雨,谭海波,黄敏仪,贝伟剑,杨祎琦..糖尿病肾病小鼠CD4+T细胞的脂质组学研究[J].中国免疫学杂志,2024,40(3):471-477,7.基金项目
广东省医学科学技术研究基金项目(B2020023). （B2020023）
