中国免疫学杂志2024，Vol.40Issue(3)：524-529,533,7.DOI:10.3969/j.issn.1000-484X.2024.03.014
美沙拉秦介导TGF-β1/Smad信号通路减轻脂多糖诱导的结肠上皮细胞炎症及凋亡
Mesalazine mediates TGF-β1/Smad signaling pathway to alleviate lipopolysac-charide-induced colonic epithelial cell inflammation and apoptosis
摘要
Abstract
Objective:To investigate effects of mesalazine(MS)on proliferation,apoptosis and inflammatory injury of cell model of ulcerative colitis(UC)induced by lipopolysaccharide(LPS),as well as transforming growth factor-β1(TGF-β1)/Smad signaling pathway effect in this study.Methods:Human colonic epithelial cells NCM-460 cultured in vitro were induced UC model by LPS,and divided into Con group(no treatment),LPS group(1 mg/L LPS),MS group(0.1,0.2,0.4 mg/L MS+1 mg/L LPS)and inhibitor group(10 µmol/L TGF-β1/Smad signaling pathway inhibitor LY2109761+0.2 mg/L MS+1 mg/L LPS).Cell morphology,proliferation,apoptosis and levels of inflammatory factors and TGF-β1/Smad pathway-related markers were examined by inverted microscope,EdU assay,Hoechst 33258 staining,ELISA and Western blot.Results:LPS treatment highly induced cell proliferation rate and Smad7 pro-tein level compared with Con group,while apoptotic cells,inflammatory factors TNF-α and IL-6,soluble interleukin-2 receptor(sIL-2R)release,as well as TGF-β1,p-Smad2,p-Smad3 protein expressions were increased;the above effects induced by LPS was reversed by MS in a dose-dependent manner(P<0.05).Compared with 0.2 mg/L MS group,NCM-460 cells proliferation rate and Smad7 expression were increased,while apoptotic cells,TNF-α and IL-6,sIL-2R releases,and TGF-β1,p-Smad2,p-Smad3 protein expressions were decreased(P<0.05).Conclusion:MS can attenuate LPS-induced apoptosis and inflammatory injury in NCM-460 cells,and this protection was possibly through suppressing TGF-β1/Smad signaling pathway.关键词
美沙拉秦/溃疡性结肠炎/增殖/凋亡/转化生长因子-βKey words
Mesalazine/Ulcerative colitis/Proliferation/Apoptosis/Transforming growth factor-β分类
医药卫生引用本文复制引用
侯静,刘加宁,冯如,陆伟,王云,苏峰..美沙拉秦介导TGF-β1/Smad信号通路减轻脂多糖诱导的结肠上皮细胞炎症及凋亡[J].中国免疫学杂志,2024,40(3):524-529,533,7.基金项目
2022年度宿迁市指导性科技计划项目(Z2022050). （Z2022050）
