中国免疫学杂志2024，Vol.40Issue(3)：540-545,6.DOI:10.3969/j.issn.1000-484X.2024.03.017
鸢尾黄素通过调节TLR4/MyD88/NF-κB信号通路改善血管性痴呆大鼠认知缺陷
Tectorigenin improves cognitive deficits in rats with vascular dementia by regulating TLR4/MyD88/NF-κB signaling pathway
摘要
Abstract
Objective:To analyze effects of tectorigenin on improving cognitive deficits in rats with vascular dementia(VD)by regulating Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear factor-κB(NF-κB)signaling pathway.Methods:A total of 72 rats were randomly divided into sham operation group,model group,low,medium and high doses[25,50,100 mg/(kg·d)]tectorigenin groups and positive control group[piracetam 324 mg/(kg·d)],with 12 rats in each group.Except for sham operation group,VD models were replicated in other groups.After successful modeling,different doses tectorigenin groups and positive control group were administered intragastrically with different doses of tectorigenin and piracetam,while other groups were administered intragastrically with same volume of normal saline for 28 d.Spatial learning and memory ability were detected by Morris water maze.Neurotransmitter levels in hippocampus interstitial fluid were detected by high performance liquid chromatography-electro-chemical.Brain-derived neurotrophic factor(BDNF)and tyrosine kinase receptor b(TrkB)expressions in hippocampus were detected by RT-qPCR and Western blot.TLR4/MyD88/NF-κB pathway-related proteins in hippocampus were detected by Western blot.Results:Compared with sham operation group,escape latency was longer,while stay time in target area and times of crossing platform were lower in model group(P<0.05).Compared with model group,escape latency was shorter,while stay time in target area and times of crossing platform were higher in medium and high doses tectorigenin groups(P<0.05).NE,DA,5-HT and 5-HIAA levels in model group were lower than those in sham operation group(P<0.05),which were higher in medium and high doses tectorigenin groups than model group(P<0.05).Compared with sham operation group,BDNF and TrkB mRNA and proteins levels were lower,while TLR4,MyD88 and p-NF-κB p65/NF-κB p65 proteins levels were higher in model group(P<0.05).Compared with model group,BDNF and TrkB mRNA and proteins levels were higher,while TLR4,MyD88 and p-NF-κB p65/NF-κB p65 proteins levels were lower in medium and high doses tectorigenin groups(P<0.05).Conclusion:Tectorigenin can improve cognitive deficits in VD rats,which may be related to regulating TLR4/MyD88/NF-κB signaling pathway.关键词
鸢尾黄素/Toll样受体4/髓样分化因子88/核因子κB信号通路/血管性痴呆/认知缺陷Key words
Tectorigenin/Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB signaling pathway/Vascular dementia/Cognitive deficit分类
医药卫生引用本文复制引用
丁旭,邓祥敏,殷紫,刘旭,谭东明,尹红英..鸢尾黄素通过调节TLR4/MyD88/NF-κB信号通路改善血管性痴呆大鼠认知缺陷[J].中国免疫学杂志,2024,40(3):540-545,6.基金项目
江苏省卫生健康委科研项目(Z2020054) （Z2020054）
江苏省高职院校教师专业带头人高端研修项目(2022GRFX016). （2022GRFX016）
