应用生态学报2024，Vol.35Issue(3)：713-720,8.DOI:10.13287/j.1001-9332.202403.015
根施生物炭对设施连作土壤氮素转化及黄瓜幼苗根系氮代谢的影响
Effects of root-applied biochar on soil nitrogen transformation and root nitrogen metabolism of cucumber seedlings in facility continuous cropping soils
摘要
Abstract
The problem of soil barrier caused by excessive accumulation of nitrogen is common in continuous crop-ping soil of facility agriculture.To investigate the modulating effects of biochar amendment on soil nitrogen transfor-mation in greenhouse continuous cropping systems,we conducted a pot experiment with two treatments,no biochar addition(CK)and 5％biochar addition(mass ratio).We analyzed the effects of biochar addition on soil microbial community structure,abundances of genes functioning in nitrogen cycling,root growth and nitrogen metabolism-re-lated genes expressions of cucumber seedlings.The results showed that biochar addition significantly increased plant height,root dry mass,total root length,root surface area,and root volume of cucumber seedlings.Rhizosphere en-vironment was improved,which enhanced root nitrogen absorption by inducing the up-regulation of genes expres-sions related to plant nitrogen metabolism.Biochar addition significantly increased soil microbial biomass nitrogen,nitrate nitrogen,and nitrite nitrogen contents.The abundances of bacteria that involved in nitrogen metabolism,in-cluding Proteobacteria,Cyanobacteria,and Rhizobiales(soil nitrogen-fixing bacteria),were also significantly im-proved in the soil.The abundances of genes functioning in soil nitrification and nitrogen assimilation reduction,and the activities of enzymes involved in nitrogen metabolisms such as hydroxylamine dehydrogenase,nitronate monoox-ygenase,carbonic anhydrase were increased.In summary,biochar addition improved soil physicochemical proper-ties and microbial community,and affected soil nitrogen cycling through promoting nitrification and nitrogen assimi-lation.Finally,nitrogen adsorption capacity and growth of cucumber plant was increased.关键词
设施连作土壤/生物炭/氮素循环/土壤微生物/宏基因组Key words
facility continuous cropping soil/biochar/nitrogen cycling/soil microorganism/macrogenome引用本文复制引用
杨雪,曹霞,白冰,袁艳娜,张宁,谢洋,武春成..根施生物炭对设施连作土壤氮素转化及黄瓜幼苗根系氮代谢的影响[J].应用生态学报,2024,35(3):713-720,8.基金项目
本文由河北省重点研发计划项目(21326901D)、河北科技师范学院省属高校基本科研业务费专项(2021JK14)和秦皇岛市现代农业产业技术体系旱黄瓜创新团队项目资助. （21326901D）
