畜牧与兽医2024，Vol.56Issue(4)：54-60,7.
刻点血蜱成蜱中肠菌群结构的分析
Analysis of the midgut microbial community structure in adult ticks of Haemaphysalis punctata
摘要
Abstract
In this study,we aimed to investigate the characteristics of and differences in the midgut microbial community structure of female and male Haemaphysalis punctata.The female and male H.punctata were collected from the body of sheep in Shawan,Xinjiang.The midgut contents of both sexes were collected under sterile conditions,the total bacterial DNA of each sample was extracted,and the bacterial 16S rDNA V3-V4 region was amplified by PCR and sequenced using Illiumina NovaSeq high-throughput double-end sequencing.After splicing and filtering the data,denoising with ASVs,and species annotation,the composition of and differences in the midgut microbial community structure in female and male H.punctata were compared and analyzed.The results showed that the diversity of midgut microbial community in the female ticks was higher than that in the male.At the phylum level,the relative abundance of Proteobacteria,Firmicutes,Deinococcota,Bacteroidota,and Actinobacteriota was high in the midgut of the female and male ticks,with Proteobacteria having the highest abundance and being the dominant phylum in the two samples.And the relative abundance of Proteobacteria in the midgut of the male ticks was higher than that of the female.At the genus level,the relative abundance of Schlegelella,Acinetobacter,Bacteroides,Lactobacillus,Brevundimonas,Romboutsia was high in the midgut of both male and female ticks,with Schlegelella having the highest abundance and being superior in the female(41.7%)compared with those in the male(0.8%).Annotated to the species level,the bacteria species common to the two samples included Meiothermus silvanus,Acinetobacter baumannii,Coxiella endosymbiont,Lactobacillus intestinalis,Bifidobacterium longum,and Bac-teroides barnesiae.The present results indicated that the midgut microbial community structure was relatively similar in both female and male H.punctata,but the relative abundance of each bacterium varied greatly at different taxonomic levels,which might be related to the different blood-sucking and physiological characteristics of female and male H.punctata.关键词
刻点血蜱/雌蜱/雄蜱/中肠/菌群结构Key words
Haemaphysalis punctata/female ticks/male ticks/midgut/microbial community分类
农业科技引用本文复制引用
刘龙江,刘潇雨,程天印,段德勇,吴平顺..刻点血蜱成蜱中肠菌群结构的分析[J].畜牧与兽医,2024,56(4):54-60,7.基金项目
国家自然科学基金青年项目(31902294) （31902294）
