畜牧与兽医2024，Vol.56Issue(4)：72-77,6.
不同基因型牛病毒性腹泻病毒对BALB/c小鼠致病性研究
Pathogenicity of different genotypes of bovine viral diarrhea virus on BALB/c mice
摘要
Abstract
Bovine viral diarrhea virus(BVDV)is an important pathogen that endangers the cattle industry,and it causes huge economic losses to the world's cattle industry.With the increase of international trade,the cattle have been transported frequently,BVDV strains con-tinue to mutate,and new gene subtypes continue to appear.In 2021,our laboratory isolated a BVDV(JS2201)from bovine lung with pneu-monia.In order to evaluate the virulence and pathogenicity of the BVDV isolate in our laboratory,we established respective BALB/c mice models infected with the BVDV isolated strain,the BVDV-1 type Oregon C24V reference strain,and BVDV-2 type C1602.By observing and detecting the clinical symptoms,weight changes,viremia,changes in white blood cells and lymphocytes in different groups of mice after challenge,the histopathological changes and viral load of various tissues and organs were compared.The results showed that both the BVDV isolate JS2201 and the reference strain were able to infect BALB/c mice successfully,and the infected BALB/c mice developed viremia,re-sulting in a temporary decrease in the number of white blood cells,lymphocytes and percentage of lymphocytes in the blood.The BVDV loads in the liver,kidney,lung and spleen of the mice infected with the BVDV isolate JS2201 strain were higher than those in the C24V and C1602 infected groups.All mice in the challenged group showed interstitial pneumonia.The pathological changes of lung in the challenged group of the JS2201 strain,and the reference strain C24V of type 1 strain were similar.The lung disease in the challenged group of the BVDV reference strain C1602 strain got more severe.In this study,the model of acute infection of BALB/c mice with different subtypes of BVDV was successfully established,and the pathogenicity difference between the isolated strain and the reference strain were compared and ana-lyzed;which laid the foundation for the development of BVDV vaccines and the study of their pathogenesis.关键词
牛病毒性腹泻病毒/BALB/c小鼠/致病性/基因型Key words
bovine viral diarrhea virus/BALB/c mice/pathogenicity/genotype分类
农业科技引用本文复制引用
刘镝钺,熊富强,刘茂军,程子龙,陈益,陈思宇,李文良,毛立,杨蕾蕾,孙敏,张纹纹..不同基因型牛病毒性腹泻病毒对BALB/c小鼠致病性研究[J].畜牧与兽医,2024,56(4):72-77,6.基金项目
国家重点研发计划项目(2022YFD1800603) （2022YFD1800603）
