浙江农林大学学报2024，Vol.41Issue(2)：223-233,11.DOI:10.11833/j.issn.2095-0756.20230385
中波紫外线对三叶青酚类物质质量分数、抗氧化能力及基因表达的影响
Effect of UV-B radiation on mass fraction of phenolic substances,antioxidant capacity and genes expression in Tetrastigma hemsleyanum
摘要
Abstract
[Objective]This study aims to investigate the effects of UV-B radiation on phenolic components,antioxidant capacity and relation genes expression in Tetrastigma hemsleyanum.[Method]Three-year-old T.hemsleyanum seedlings were subjected to continuous UV-B radiation for 12.0 h and their phenotypes were observed at 0,0.5,1.0,3.0,6.0,and 12.0 h.With those untreated as control(ck),some other T.hemsleyanum were irradiated for 1.0 and 3.0 h(T1 and T3),and supplemented with dark treatment for 24.0 h(T1+23 and T3+21).Mass fractions of phenolic substances(total phenols and total flavonoids),antioxidant capacity,anti-stress enzyme activity,malondialdehyde(MDA)mass molar concentration in leaves were determined and transcriptome analysis was made.[Result]Continuous UV-B radiation had an effect on the phenotype of T.hemsleyanum.The leaves of 3-h group were partially yellowed,while the leaves of 6-h and 12-h groups were significantly yellowed and slightly curled.The mass fractions of total phenols(2.65 and 2.63 mg·g-1)and total flavonoids(7.16 and 7.30 mg·g-1)in leaves and root tubers,as well as the antioxidant capacity(86.56%)in root tubers reached the maximum in T1+23 group.Meanwhile,dark treatment after UV-B radiation promoted a significant increase in activities of catalase(CAT),peroxidase(POD)and superoxide dismutase(SOD),which reached the maximum in T1+23 group.The MDA mass molar concentration was the highest in T3+21 group(7.21 mmol·g-1).UV-B radiation and dark treatment down-regulated the expression of flavonoid synthesis pathway genes(ThF3H,ThANS,ThCHS,ThANR,etc.)and up-regulated the expression of oxidation-related regulators(CAT and SOD).[Conclusion]Darkness treatment after UV-B radiation increases the mass fraction of phenolic substances,antioxidant capacity and anti-stress enzymes activity of T.hemsleyanum,reaching the maximum in T1+23 group.The expression of flavonoid synthase genes is down-regulated and the expression of oxidative stress-related genes is up-regulated.[Ch,7 fig.2 tab.52 ref.]关键词
三叶青/中波紫外线胁迫/多酚类物质/抗氧化能力/差异表达Key words
Tetrastigma hemsleyanum/UV-B stress/phenolic substances/antioxidant capacity/differential expression分类
农业科技引用本文复制引用
夏敬青,刘海荣,顾依雯,王子玥,邢巧月,张宇翔,李姗,白岩..中波紫外线对三叶青酚类物质质量分数、抗氧化能力及基因表达的影响[J].浙江农林大学学报,2024,41(2):223-233,11.基金项目
浙江省"尖兵""领雁"研发攻关计划项目(2023C02054) （2023C02054）
浙江农林大学发展基金资助项目(2022LFR111) （2022LFR111）
