浙江农林大学学报2024，Vol.41Issue(2)：275-285,11.DOI:10.11833/j.issn.2095-0756.20230342
闽楠bZIP基因家族鉴定和脱落酸处理下的表达分析
Identification and expression analysis of bZIP gene family under ABA treatment in Phoebe bournei
摘要
Abstract
[Objective]This study is aimed to identify the bZIP transcription factor family members from Phoebe bournei and investigate the response levels of its members to abscisic acid(ABA)treatment.[Method]A bioinformatic method was employed to identify the PbbZIPs family throughout the whole genome and to analyze its physicochemical properties,gene structure,evolutionary relationships,cis-acting elements in promoter,and the expression patterns under ABA treatment by RT-qPCR.[Result]A total of 63 PbbZIP genes were identified from 12 chromosomes in P.bournei,divided into 12 subfamilies with significantly different in gene structure and motifs,but highly conserved in the same subfamily.Most of the PbbZIPs were localized in the nucleus,and their encoded protein were 110-835 amino acid in length,-1.19--0.19 in hydrophobicity,and 4.48-11.95 in isoelectric point.The 27 pairs of PbbZIPs distributed on 12 chromosomes were featured with collinearity existence,which was the main pattern of PbbZIPs family expansion.A variety of abiotic stress-related action elements were found in the upstream promoter region of PbbZIPs,among which ABA,salicylic acid and methyl jasmonate were more abundant response elements and the genes expression of RT-qPCR revealed that 17 PbbZIPs were induced differentially by ABA signals and generally up-regulated in leaves and roots when P.bournei was treated with 2 mmol·L-1ABA for 1-72 h,with the relative expression of PbbZIPs in roots being generally lower than that in leaves.[Conclusion]The 63 PbbZIPs identified from the P.bournei genome were unevenly distributed across the 12 chromosomes and highly similar in gene sequences,whereas the chromosomal localization genetic structure and conserved motifs were evolutionarily diverse and different between subgroups.The PbbZIPs in leaves and roots responded differently to ABA treatment and got involved in the regulation of other abiotic processes.[Ch,9 fig.35 ref.]关键词
闽楠/bZIP家族/系统进化/脱落酸(ABA)/表达分析Key words
Phoebe bournei/bZIP family/systematic evolution/ABA/expression analysis分类
农业科技引用本文复制引用
杨勇,张俊红,韩潇,张毓婷,杨琪,童再康..闽楠bZIP基因家族鉴定和脱落酸处理下的表达分析[J].浙江农林大学学报,2024,41(2):275-285,11.基金项目
浙江省农业新品种选育重大专项(2021C02070-10) （2021C02070-10）
国家自然科学基金资助项目(32171828) （32171828）
